JCEG ID JCEG083
Name gly-12      WormBase    NCBI Gene
Human Ortholog

GGDB:MGAT1 

OMIM: 160995

OrthoList :   MGAT1  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
DOWNSTREAM 0.85 0.000006 (females) 0.16 (kg decrease) [NR] 19851299
DOWNSTREAM 0.85 0.00000007 (females) 2.96 (kg decrease) [NR] 19851299
MAPPED 0.47 0.000005 (HDL-C) 0.23 (mg/dL increase) [NR] 19197348
DOWNSTREAM 0.14 0.000008 (EA-glucose response) 3.67 (mg/dL increase) [2.06-5.28] 23400010
GGDB Score E-val
MGAT1 220 5e-59
POMGNT1 115 1e-27
MGAT1 219 1e-58
POMGNT1 114 3e-27
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcNAcT(GnT)
Locus Tag F48E3.1
RNAi phenotype
phenotype Emb
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
dxd:EDD
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Chen S, Zhou S, Sarkar M, Spence AM, Schachter H J. Biol. Chem. (1999) 274 (1) :288-97 [PMID : 9867843] Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development.

Maeda I, Kohara Y, Yamamoto M, Sugimoto A Curr. Biol. (2001) 11 (3) :171-6 [PMID : 11231151] Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi.

Chen S, Tan J, Reinhold VN, Spence AM, Schachter H Biochim. Biophys. Acta (2002) 1573 (3) :271-9 [PMID : 12417409] UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans.

Zhang W, Cao P, Chen S, Spence AM, Zhu S, Staudacher E, Schachter H Biochem. J. (2003) 372 (Pt 1) :53-64 [PMID : 12603202] Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.

Paschinger K, Rendic D, Lochnit G, Jantsch V, Wilson IB J. Biol. Chem. (2004) 279 (48) :49588-98 [PMID : 15364955] Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans.

Zhu S, Hanneman A, Reinhold VN, Spence AM, Schachter H Biochem. J. (2004) 382 (Pt 3) :995-1001 [PMID : 15228383] Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Shi H, Tan J, Schachter H Meth. Enzymol. (2006) 417 :359-89 [PMID : 17132514] N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens.

Houston KM, Sutharsan R, Steiger CN, Schachter H, Harnett W Mol. Biochem. Parasitol. (2007) 157 (1) :88-91 [PMID : 17920136] Gene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis.

Meissner B, Warner A, Wong K, Dube N, Lorch A, McKay SJ, Khattra J, Rogalski T, Somasiri A, Chaudhry I, Fox RM, Miller DM, Baillie DL, Holt RA, Jones SJ, Marra MA, Moerman DG PLoS Genet. (2009) 5 (6) :e1000537 [PMID : 19557190] An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Chen S, Spence AM, Schachter H Biochimie 85 (3-4) :391-401 [PMID : 12770777] Isolation of null alleles of the Caenorhabditis elegans gly-12, gly-13 and gly-14 genes, all of which encode UDP-GlcNAc: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I activity.

WormBase (WS254)

Functional Descriptions gly-12

gly-12 encodes an experimentally verified UDP-N-acetylglucosamine alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I), expressed throughout development in many cell types; a deletion allele of gly-12 is phenotypically normal even as a double or triple mutant with gly-13 and gly-14.
Deletion Mutants id47 is47 ok1026
In Situ In Situ   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003679

neuron "Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses." [ISBN:0140512888] WB:Expr6110 WBPaper00006525 WormAtlas
WB WBbt:0003679

neuron "Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses." [ISBN:0140512888] Enriched WBPaper00030839 : Embryo_Pan_Neuronal, Larval_Pan_Neuronal
 WBPaper00030839 WormAtlas
WB WBbt:0004740

I5 neuron "Neuron class of one pharyngeal interneuron, posterior sensory." [ISBN:0-87969-307-X] Enriched WBPaper00025141 : unc-4
 WBPaper00025141
WB WBbt:0005135

head ganglion "ganglion in the head." [WB:rynl] Certain WB:Expr649 WBPaper00003352
WB WBbt:0005212

dorso-rectal ganglion "the ganglion that lies above and behind the rectum in the tail, in close continuity with the anal hypodermal ridge. It contains 3 neuron cell bodies (DVA, DVB and DVC) that send their neuronal processes into the ventral nerve cord via dorso-rectal commissures that encircle the anus. The ganglion contains no local neuropil in the hermaphrodite. In the adult male tail, this ganglion gains additional neurons and some local neuropil. " [WBPaper:00038216] Certain WB:Expr649 WBPaper00003352 WormAtlas
WB WBbt:0005278

DA neuron "Neuron class of nine ventral cord motor neuron, innervates dorsal muscle." [WB:rynl] Enriched WBPaper00025141 : unc-4
 WBPaper00025141
WB WBbt:0005278

DA neuron "Neuron class of nine ventral cord motor neuron, innervates dorsal muscle." [WB:rynl] Enriched WBPaper00030839 : Embryo_A_Class
 WBPaper00030839
WB WBbt:0005300

ventral cord neuron "neuron with cell body associated with the ventral nerve cord." [WB:Paper00000938] WB:Expr6110 WBPaper00006525 WormAtlas
WB WBbt:0005396

SAB "Neuron class of three interneurons, which send processes anteriorly up the sub-lateral cords in the head." [WB:Paper00000938] Enriched WBPaper00025141 : unc-4
 WBPaper00025141
WB WBbt:0005406

ALM "Neuron class of two anterior sensory neurons that transduce touch stimuli." [WB:Paper00000938] Enriched WBPaper00040420 : ALM_PLM_enriched
 WBPaper00040420
WB WBbt:0005448

preanal ganglion "ganglion anterior to the anus." [WB:rynl] Certain WB:Expr649 WBPaper00003352 WormAtlas
WB WBbt:0005490

PLM "Neuron class of two posterior sensory neurons that transduce touch stimuli." [WB:Paper00000938] Enriched WBPaper00040420 : ALM_PLM_enriched
 WBPaper00040420
WB WBbt:0005656

retrovesicular ganglion "ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.\nReversal \n\n" [WBPaper:00038216] Enriched WBPaper00025141 : unc-4
 WBPaper00025141 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Certain WB:Expr649 WBPaper00003352
WB WBbt:0005735

nervous system "Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal's internal and external environments and to initiate and integrate its effector responses and activities." [ISBN:0-14-051288-8, WB:rynl] WB:Expr6110 WBPaper00006525 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] WB:Expr6110 WBPaper00006525 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr649 WBPaper00003352 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00029359 WormAtlas
WB WBbt:0005813

body wall musculature "Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion." [ISBN:0-87969-433-5] Certain WB:Expr649 WBPaper00003352 WormAtlas
WB WBbt:0005813

body wall musculature "Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion." [ISBN:0-87969-433-5] Enriched WBPaper00040985 : AIN-2_asynchronous_muscle
 WBPaper00040985 WormAtlas
WB WBbt:0005830

lumbar ganglion "The left and right lumbar ganglia lie behind the pre-anal ganglion in the tail, in lateral positions. Each contains 12 neuron cell bodies that send most of their neuronal processes into the ventral nerve cord via lumbar commissures and the pre-anal ganglion. There is no local neuropil in these ganglia in the hermaphrodite, instead they form most synapses in the pre-anal ganglion. Most lumbar neurons have sensory functions. In the adult male tail, these two ganglia gain many additional neurons and have some local neuropil. The lumbar neurons lie in close association with the lateral hypodermis, and most are posterior to the anus." [WBPaper:00038216] Certain WB:Expr649 WBPaper00003352 WormAtlas
WB WBbt:0006749

nerve ring "the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes." [WB:Paper00000938] WB:Expr6110 WBPaper00006525 WormAtlas
WB WBbt:0006751

head neuron "neuron with its cell body situated in the head, excluding the pharynx." [WB:rynl] WB:Expr6110 WBPaper00006525
WB WBbt:0006759

tail neuron "neuron with its cell body situated in the tail, posterior to rectum." [WB:rynl] WB:Expr6110 WBPaper00006525
WB WBbt:0006828

FLP "Neuron class of two neurons, which have ciliated endings situated immediately dorsal to the lateral inner labial sensilla in the head but have no associated sheath or socket cells; they have flattened processes in this region of the cilium." [WB:Paper00000938] Enriched WBPaper00040420 : FLP_enriched
 WBPaper00040420

WBGene00001637
Phenotype
Homology_paralog
Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00000768   WBPaper00004651   Observed


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