JCEG ID JCEG090
Name cgt-3      WormBase    NCBI Gene
Human Ortholog

GGDB:UGCG 

OMIM: 602874

OrthoList :   UGCG  (Ensembl Compara)

GGDB Score E-val
UGCG 347 3e-97
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcT
Locus Tag F59G1.1
RNAi phenotype
phenotype
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Ichikawa S, Hirabayashi Y Trends Cell Biol. (1998) 8 (5) :198-202 [PMID : 9695839] Glucosylceramide synthase and glycosphingolipid synthesis.

Leipelt M, Warnecke D, Zahringer U, Ott C, Muller F, Hube B, Heinz E J. Biol. Chem. (2001) 276 (36) :33621-9 [PMID : 11443131] Glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants, and fungi.

Lochnit G, Bongaarts R, Geyer R Int. J. Parasitol. (2005) 35 (8) :911-23 [PMID : 15885697] Searching new targets for anthelminthic strategies: Interference with glycosphingolipid biosynthesis and phosphorylcholine metabolism affects development of Caenorhabditis elegans.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Marza E, Simonsen KT, Faergeman NJ, Lesa GM J. Cell. Sci. (2009) 122 (Pt 6) :822-33 [PMID : 19240113] Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells.

Zhang H, Abraham N, Khan LA, Hall DH, Fleming JT, Gobel V Nat. Cell Biol. (2011) 13 (10) :1189-201 [PMID : 21926990] Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.

Kao CY, Los FC, Huffman DL, Wachi S, Kloft N, Husmann M, Karabrahimi V, Schwartz JL, Bellier A, Ha C, Sagong Y, Fan H, Ghosh P, Hsieh M, Hsu CS, Chen L, Aroian RV PLoS Pathog. (2011) 7 (3) :e1001314 [PMID : 21408619] Global functional analyses of cellular responses to pore-forming toxins.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K Glycobiology (2011) 21 (6) :834-48 [PMID : 21325339] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.

WormBase (WS254)

Functional Descriptions cgt-3

cgt-3 encodes a transmembrane protein that is one of three C. elegans ceramide glucosyltransferases; by homology, CGT-3 is predicted to localize to the Golgi membrane and catalyze the transfer of glucose from UDP-glucose to ceramide to synthesize glucosylceramide, a glycosphingolipid precursor; although cgt-3 mutant worms show no obvious defects, animals doubly mutant for cgt-3 and cgt-1 arrest development in the early L1 larval stage, showing impaired feeding ability; in addition, cgt-3;cgt-1 and cgt-3;cgt-1;cgt-2 mutant animals show reduced glucosylceramide levels; a CGT-3::GFP reporter fusion is expressed in pharyngeal tissues, the intestine, the pharyngeal-intestinal and intestinal-rectal valves, the rectal gland cells, head mesodermal cells and additional unidentified head cells; cgt-3 expression in mouse GM95 cells, deficient in glucosylceramide due to a defect in glucosylceramide synthase, confirms that CGT-3 has ceramide glucosyltransferase activity.
Deletion Mutants ok610 ok2877 tm504
In Situ In Situ   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003681

pharynx "the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine." [WB:rynl] WB:Expr6269 WBPaper00006525 WormAtlas
WB WBbt:0003681

pharynx "the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine." [WB:rynl] Certain WB:Expr8290 WBPaper00028914 WormAtlas
WB WBbt:0004510

virR "Intestino-rectal valve" [ISBN:0-87969-307-X] Certain WB:Expr8572 WBPaper00032934 WormAtlas
WB WBbt:0004511

virL "Intestino-rectal valve" [ISBN:0-87969-307-X] Certain WB:Expr8572 WBPaper00032934 WormAtlas
WB WBbt:0004697

hmc "Head mesodermal cell, function unknown" [ISBN:0-87969-307-X] WB:Expr6269 WBPaper00006525
WB WBbt:0004697

hmc "Head mesodermal cell, function unknown" [ISBN:0-87969-307-X] Certain WB:Expr8572 WBPaper00032934
WB WBbt:0005319

spermatheca "an accordion-like tube that contains sperm and is the site of oocyte fertilization." [WBPaper:00038216] Certain WB:Expr8290 WBPaper00028914 WormAtlas
WB WBbt:0005451

pharyngeal muscle cell "type of cells that make up muscle layers in the pharynx." [WB:rynl] Certain WB:Expr8572 WBPaper00032934 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Certain WB:Expr11539 WBPaper00038174
WB WBbt:0005741

tail "posterior region, from rectum to the end" [WB:rynl] Partial WB:Expr8290 WBPaper00028914 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] WB:Expr6269 WBPaper00006525 WormAtlas
WB WBbt:0005767

pharyngeal-intestinal valve "A group of six equivalent cells forms a tightly constructed 'valve' that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine." [WBPaper:00038216] Certain WB:Expr8572 WBPaper00032934 WormAtlas
WB WBbt:0005767

pharyngeal-intestinal valve "A group of six equivalent cells forms a tightly constructed 'valve' that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine." [WBPaper:00038216] Certain WB:Expr11539 WBPaper00038174 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] WB:Expr6269 WBPaper00006525 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr8290 WBPaper00028914 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr8572 WBPaper00032934 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00040985 : AIN-2_L4_intestine, AIN-2_asynchronous_intestine
 WBPaper00040985 WormAtlas
WB WBbt:0005784

germ line "cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes." [ISBN:0140512888] Certain WB:Expr11539 WBPaper00038174
WB WBbt:0005797

rectal valve cell "cell that forms intestinal-rectal valve." [WB:rynl] Certain WB:Expr11539 WBPaper00038174 WormAtlas
WB WBbt:0005799

rectal gland cell Certain WB:Expr8572 WBPaper00032934 WormAtlas

WBGene00019127
Phenotype
Homology_paralog
Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
Bacillus thuringiensis toxin hypersensitive

"Animals respond to Bacillus thuringiensis toxins (Bt toxins) at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557]
RNAi: WBRNAi00088628   WBPaper00038231   Observed
RNAi: WBRNAi00088735   WBPaper00038231   Observed
feeding inefficient

"Animals are unable to ingest nutrients at a rate similar to control animals." [WB:WBPaper00032342, WB:WBPerson712]
RNAi: WBRNAi00078081   WBPaper00032934   Observed
intestinal morphology variant

"Animals exhibit variations in the form, structure or composition of the chain of cuboidal cells which form a central lumen lined by microvilli that allow the passage of food, compared to control. Food passes from the posterior pharynx to the intestine where it is digested and then on to the rectum which processes the waste products for excretion (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00078082   WBPaper00032934   Observed
L1 lethal

"Animals die at the L1 stage of the life cycle." [WB:WBPerson2021]
RNAi: WBRNAi00078076   WBPaper00032934   Observed
RNAi: WBRNAi00078080   WBPaper00032934   Observed
organism metabolism processing variant

"Variations in the chemical reactions and pathways, including anabolism and catabolism, by which living organisms transform chemical substances compared to control." [GO:0008152, WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00078078   WBPaper00032934   Observed
RNAi: WBRNAi00078079   WBPaper00032934   Observed
pore forming toxin hypersensitive

"Animals respond to pore forming toxins at a lower concentration or a shorter exposure compared to control animals. Increased susceptibility to pore forming toxins (e.g. Cry5B from Bacillus thuringiensis) is often measured by a decrease in the rate of survival in the presence of pore forming toxins compared to controls." [WB:WBPerson2987, WB:WBPerson557]
RNAi: WBRNAi00088628   WBPaper00038231   Observed
RNAi: WBRNAi00088735   WBPaper00038231   Observed
reduced brood size

"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557]
RNAi: WBRNAi00083974   WBPaper00025177   Observed
RNAi: WBRNAi00083996   WBPaper00025177   Observed


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