JCEG ID JCEG046
Name pad-2      WormBase    NCBI Gene
Human Ortholog

GGDB:POFUT2 

OMIM: 610249

OrthoList :   POFUT2  (Ensembl Compara)

GGDB Score E-val
POFUT2 337 2e-94
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name Fucosyltransferases
Locus Tag K10G9.3
RNAi phenotype
phenotype Emb
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features tm:Detected
gg_motif:M1,M2,M3,M4,M5,M6
kr:Detected
Sequence CDS
Protein
References

Menzel O, Vellai T, Takacs-Vellai K, Reymond A, Mueller F, Antonarakis SE, Guipponi M Genomics (2004) 84 (2) :320-30 [PMID : 15233996] The Caenorhabditis elegans ortholog of C21orf80, a potential new protein O-fucosyltransferase, is required for normal development.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Kozma K, Keusch JJ, Hegemann B, Luther KB, Klein D, Hess D, Haltiwanger RS, Hofsteenge J J. Biol. Chem. (2006) 281 (48) :36742-51 [PMID : 17032646] Identification and characterization of abeta1,3-glucosyltransferase that synthesizes the Glc-beta1,3-Fuc disaccharide on thrombospondin type 1 repeats.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

WormBase (WS254)

Functional Descriptions pad-2

pad-2 encodes an O-fucosyltransferase; by homology, PAD-2 is predicted to catalyze the transfer of fucose to serine or threonine residues on recipient proteins; RNAi experiments indicate that pad-2 activity is required for embryogenesis, particularly morphogenesis and late embryonic patterning, and for various aspects of post-embryonic development including neuronal, body wall muscle, and vulval development as well as spermatozoid differentiation; pad-2 expression begins during embryonic morphogenesis and continues through adulthood; pad-2 is widely expressed in tissues such as hypodermis, neurons, intestine, somatic gonad, and tissues surrounding the vulva.
Deletion Mutants tm1756
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005319

spermatheca "an accordion-like tube that contains sperm and is the site of oocyte fertilization." [WBPaper:00038216] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0005394

amphid neuron "neuron of the amphid sensillum" [WB:rynl] WB:Expr6383 WBPaper00006525 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Partial WB:Expr3023 WBPaper00024260
WB WBbt:0005735

nervous system "Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal's internal and external environments and to initiate and integrate its effector responses and activities." [ISBN:0-14-051288-8, WB:rynl] WB:Expr6383 WBPaper00006525 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] WB:Expr6383 WBPaper00006525 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0005799

rectal gland cell WB:Expr6383 WBPaper00006525 WormAtlas
WB WBbt:0005812

excretory cell "H-shaped cell associated with the excretory system, largest cell in C. elegans." [WB:rynl] WB:Expr6383 WBPaper00006525 WormAtlas
WB WBbt:0005813

body wall musculature "Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion." [ISBN:0-87969-433-5] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0005828

gonadal sheath cell "five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis." [WBPaper:00038216] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0005829

ventral nerve cord "a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring." [WB:Paper00000938] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0006748

vulva "female genital." [WB:rynl] Uncertain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0006749

nerve ring "the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes." [WB:Paper00000938] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0006750

dorsal nerve cord "a bundle of nerve processes that runs along the dorsal mid-line of the animal." [WB:Paper00000938] Certain WB:Expr3023 WBPaper00024260 WormAtlas
WB WBbt:0006751

head neuron "neuron with its cell body situated in the head, excluding the pharynx." [WB:rynl] WB:Expr6383 WBPaper00006525

WBGene00010757
Phenotype
Homology_paralog
Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
body wall muscle morphology variant

"Any variation in the morphological appearance of the body wall muscle compared to control animals." [WB:WBPerson557]
RNAi: WBRNAi00062873   WBPaper00024260   Observed
body wall muscle sarcomere morphology variant

"Any variation in the form, structure or composition of the repeating units of a myofibrils in a muscle cell that are composed of an array of overlapping thick and thin filaments, compared to control." [GO:0030017, WB:WBPerson557]
RNAi: WBRNAi00062873   WBPaper00024260   Observed
egg laying variant

" Animals exhibit variations in the stage of eggs laid, egg laying cycle, number of eggs or egg laying in response to stimuli compared to control." [pmid:11813735, pmid:9697864, WB:cab]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
epithelial morphology variant

"Variations in the form, composition or structure of the layer of cells that cover the external surface of an organism and form the inner lining between the tissues and organs of that organism and the external environment compared to control. In C. elegans epithelial cells include epithelium of the alimentary tract, intestinal cells, hypodermis, interfacial cells and somatic gonad cells (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00062874   WBPaper00024260   Observed
everted vulva

"The eversion process, in which the vulval invagination is turned inside out during the final molt, is disrupted. In C.elegans, such variations in vulval eversion are primarily due to developmental defects in the somatic gonad (and sometimes vulva)." [WB:cab, WB:WBPaper00001738, WB:WBPerson2021, WB:WBPerson261]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
gut granule development variant

"Any variation in the progression of the different inclusions within the intestinal cytoplasm, including birefringent crystalline objects and other dark-staining endosomes over time compared to control. In C. elegans these contents might include glycogen granules (polysaccharide storage), rhabditin (carbohydrate storage), yolk granules (lipid and protein), and concentrated waste products (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
neuron morphology variant

"Animals display variations in the structure or organization of components of the neuronal cell internal to the cell itself or in relation to the cellular environment compared to control. A neuron is a major cell type of nervous tissue specialized for transmission of information in the form of patterns of impulses." [WB:cab, WB:WBPerson712]
RNAi: WBRNAi00062872   WBPaper00024260   Observed
organism morphology variant

"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
spermatogenesis variant

"Any variation in the processes that influence the generation and maturation of spermatozoa (motile sperm) compared to control." [GO:0007283, WB:WBPaper00027300, WB:WBPerson2021]
RNAi: WBRNAi00062871   WBPaper00024260   Observed
ventral nerve cord development variant

"Variations in any process whose specific outcome is the development and/or maintenance of the ventral nerve cord." [WB:WBPerson557]
RNAi: WBRNAi00062872   WBPaper00024260   Observed


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