Preparation of Membrane Rafts

Clonal analysis of Notch O-fucosylation in Drosophila melanogaster

Electrophysiological recording in the Drosophila larval muscle

RNAi-mediated knocking down of gene activities in the nematode Caenorhabditis elegans by using the Ahringer’s feeding RNAi library

Immunological analysis of glycosylation-related proteins in Drosophila tissues

Assay of Nucleotide Sugar Transport Activity (Golgi-ER transporter)

Assay of lysosome sialic acid transporter

Cloning and transfection of lysosome sialic acid transporter gene

Analysis of intracellular nucleotide sugars

Cloning and expression of nucleotide sugar or PAPS transporter genes

Assay of PAPS Transport Activity

Behavioral analysis in Drosophila melanogaster