Drosophila melanogaster is useful model organism for studying on biology. A variety of methods for analyzing a various kinds of phenotypes are developed. Here, we introduce easy behavioral assays for evaluating the function of nervous or muscular system. |
Category | Glycogene transgenic animals |
Protocol Name | Behavioral analysis in Drosophila melanogaster |
Authors
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Ueyama, Morio
Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University
Nishihara, Shoko
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Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University
*To whom correspondence should be addressed.
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KeyWords |
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Reagents
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Instruments
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1 mL-Syringe (58 mm tall and 6 mm in diameter: we use TERMO® syringe. Ca.#SS-01T) |
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Glass measuring cylinder (2000 mL) |
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Glass vials (240 mm tall and 25 mm in diameter) |
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Methods |
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Put a fly in the syringe gently (see Comment). |
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After a 5-min rest, score the number of times the fly crossed the center (0.5 mL mark) of the syringe in the next 5 min. |
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Perform 10–20 individuals, and calculate the average times the fly crossed. |
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2. |
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Dump twenty individual flies through a funnel into a glass cylinder of which inside surface is coated with mineral oil. |
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Record the scores individually, and calculate the scores average from 5 independent experiments (see Comment). |
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3. |
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Introduce twenty individual flies into a glass vial gently (see Comment). |
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After a 5-min rest, tap the bottom of the vial. |
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Record the height reached in 10 sec by a digital camera. |
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Perform five trials in each experiment, and calculate climbing ability (average ± standard error) from the 5 trials. |
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Notes | The flies are reared at 25 degree using a cornmeal-yeast-glucose standard medium under natural light conditions. Adult flies are collected within 8 h of eclosion. Every two days, adult flies are transferred a fresh food vial. All behavioral experiments should be done during daytime. |
Figure & Legends |
Figure & Legends
Fig. 1. Tools for locomotion assay
Fig. 2. Tools for flight assay |
Copyrights |
Attribution-Non-Commercial Share Alike
This work is released underCreative Commons licenses
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Date of registration:2014-05-19 13:46:43 |
- Kulkarni, S. J., and Hall, J. C. (1987) Behavioral and cytogenetic analysis of the cacophony courtship song mutant and interacting genetic variants in Drosophila melanogaster. Genetics 115, 461-475. [PMID : 3106148]
- Friggi-Grelin, F., Coulom, H., Meller, M., Gomez, D., Hirsh, J., and Birman, S. (2003) Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase. J. Neurobiol. 54, 618-627. [PMID : 12555273]
- Stockinger, P., Kvitsiani, D., Rotlopf, S., Tirian, L., and Dickson, B. J. (2005) Neural circuitry that governs Drosophila male courtship behavior. Cell 121, 795-807. [PMID : 15935765]
- Ueyama, M., Akimoto, Y., Ichimiya, T., Ueda, R., Kawakami, H., Aigaki, T., and Nishihara, S. (2010) Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome. PLoS ONE 5, e11557. [PMID : 20644630]
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How to Cite this Work in an article:
Ueyama, Morio,
Nishihara, Shoko,
(2014). GlycoPOD https://jcggdb.jp/GlycoPOD.
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How to Cite this Work in Website:
Ueyama, Morio,
Nishihara, Shoko,
(2014).
Behavioral analysis in Drosophila melanogaster.
Retrieved 2,5,2024 ,
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Ueyama, Morio,
Nishihara, Shoko,
(2014).
<b>Behavioral analysis in <em>Drosophila melanogaster</em></b>.
Retrieved 5 2,2024 ,
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