JCEG ID | JCEG176 | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Name | bus-17 WormBase NCBI Gene | ||||||||||||||||||||||||||||||||||||||||||||||||||
Human Ortholog |
GGDB:B3GALT2 OMIM:
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Family Name | b3GalT1/b3GnT orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
Locus Tag | ZK678.8 | ||||||||||||||||||||||||||||||||||||||||||||||||||
RNAi phenotype |
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Sequence Features |
tm:soluble dxd:NDD gg_motif:M1,M2 |
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Sequence | CDS |
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Protein |
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References |
Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum. Yook K, Hodgkin J Genetics (2006) 175 (2) :681-97 [PMID : 17151260] Mos1 mutagenesis reveals a diversity of mechanisms affecting response of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum. Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms. Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans. Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans. |
Functional Descriptions |
bus-17 bus-17 encodes a putative GT13 glycosyltransferase whose homologs include human B3GALT2 and C. elegans BRE-5; BUS-17 is required for bacterial infection, as well as for cuticle integrity, long-term survival, and traction during movement; specifically, bus-17 is needed both for colonization by Microbacterium nematophilum (and the tail-swelling response to M. nematophilum infection) and for attachment of Yersinia pseudotuberculosis biofilms; bus-17 is also needed for normal cuticle antigens; BUS-17 is predicted to have an N-terminal signal sequence and a moderately conserved active site; bus-17 and bus-3 function together in vivo, since bus-17(e2695)/+ sometimes shows nonallelic noncomplementation with bus-3(e2696)/+; bus-17 males are sometimes wild-type if their fathers were wild-type. |
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Deletion Mutants | br2 tm1911 | |||||||||||||||||
In Situ | ||||||||||||||||||
Expression |
Expression
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WBGene00044630 Phenotype Homology_paralog |
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Phenotype | Definition | RNAi / Allele | ||||||||||||||||
bacterially unswollen
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"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735] |
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biofilm absent head
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"Animals can move thorough a lawn of bacterially produced biofilm without accumulating an enormous amount of biofilm on its nose. When C. elegans is exposed to certain bacteria (e.g., Y. pestis), a biofilm accumulates on a worm's head. The presence of this biofilm inhibits feeding by the worm, and thereby prevents growth." [WB:cab, WB:WBPaper00005238] |
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bleach hypersensitive
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"Animals respond to bleach at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557] |
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cuticle carbohydrate surface variant
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"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712] |
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cuticle fragile
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"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712] |
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drug hypersensitive
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"Animals respond to a specific drug at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557] |
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locomotion variant
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"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712] |
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organism hypersensitive desication
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"Animals respond to less severe drought-like conditions or a shorter exposure compared to control animals." [WB:WBPerson2021] |
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organism pathogen response variant
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"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712] |
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pathogen adherence defect
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"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021] |
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pathogen resistance increased
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"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557] |
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post hermaphrodite contact variant
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"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557] |
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skiddy
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"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735] |
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slow growth
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"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557] |
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small
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"Animals are shorter and thinner than control animals at the same developmental stage." [WB:cab, WB:cgc31] |
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surface antigen negative
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"Cuticles or cuticular extracts do not bind antibodies that are observed to bind the surfaces or cuticular extracts of Bristol N2 animals of the same developmental stage." [WB:WBPaper00001280, WB:WBPerson712] |
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