JCEG ID | JCEG097 | ||||||||||||
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Name | sqv-8 WormBase NCBI Gene | ||||||||||||
Human Ortholog |
OMIM: 151290 245600 606374 607497 OrthoList : B3GAT3 B3GAT2 B3GAT1 (Ensembl Compara)
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Family Name | Glucuronyltransferase(GlcAT) | ||||||||||||
Locus Tag | ZK1307.5 | ||||||||||||
RNAi phenotype |
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Sequence Features |
kr:Detected tm:Detected dxd:DDD gg_motif:M1, M2, M3, M4, M5, M6 |
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Sequence | CDS |
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Protein |
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References |
Sigurdson DC, Spanier GJ, Herman RK Genetics (1984) 108 (2) :331-45 [PMID : 6500256] Caenorhabditis elegans deficiency mapping. Herman T, Horvitz HR Cold Spring Harb. Symp. Quant. Biol. (1997) 62 :353-9 [PMID : 9598369] Mutations that perturb vulval invagination in C. elegans. Herman T, Hartwieg E, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (1999) 96 (3) :968-73 [PMID : 9927677] sqv mutants of Caenorhabditis elegans are defective in vulval epithelial invagination. Herman T, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (1999) 96 (3) :974-9 [PMID : 9927678] Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway. Bulik DA, Wei G, Toyoda H, Kinoshita-Toyoda A, Waldrip WR, Esko JD, Robbins PW, Selleck SB Proc. Natl. Acad. Sci. U.S.A. (2000) 97 (20) :10838-43 [PMID : 11005858] sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis. Michaux G, Legouis R, Labouesse M Gene (2001) 277 (1-2) :83-100 [PMID : 11602346] Epithelial biology: lessons from Caenorhabditis elegans. Johnson TE, Henderson S, Murakami S, de Castro E, de Castro SH, Cypser J, Rikke B, Tedesco P, Link C J. Inherit. Metab. Dis. (2002) 25 (3) :197-206 [PMID : 12137228] Longevity genes in the nematode Caenorhabditis elegans also mediate increased resistance to stress and prevent disease. Hwang HY, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (2002) 99 (22) :14224-9 [PMID : 12391315] The Caenorhabditis elegans vulval morphogenesis gene sqv-4 encodes a UDP-glucose dehydrogenase that is temporally and spatially regulated. Bulik DA, Robbins PW Biochim. Biophys. Acta (2002) 1573 (3) :247-57 [PMID : 12417407] The Caenorhabditis elegans sqv genes and functions of proteoglycans in development. Sugahara K, Mikami T, Uyama T, Mizuguchi S, Nomura K, Kitagawa H Curr. Opin. Struct. Biol. (2003) 13 (5) :612-20 [PMID : 14568617] Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate. Mizuguchi S, Uyama T, Kitagawa H, Nomura KH, Dejima K, Gengyo-Ando K, Mitani S, Sugahara K, Nomura K Nature (2003) 423 (6938) :443-8 [PMID : 12761550] Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. Sternberg PW WormBook (2005) :1-28 [PMID : 18050418] Vulval development. Sato K, Sato M, Audhya A, Oegema K, Schweinsberg P, Grant BD Mol. Biol. Cell (2006) 17 (7) :3085-94 [PMID : 16672374] Dynamic regulation of caveolin-1 trafficking in the germ line and embryo of Caenorhabditis elegans. Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation. Franks DM, Izumikawa T, Kitagawa H, Sugahara K, Okkema PG Dev. Biol. (2006) 296 (2) :409-20 [PMID : 16828468] C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans. Hadwiger G, Dour S, Arur S, Fox P, Nonet ML PLoS ONE (2010) 5 (4) :e10161 [PMID : 20405020] A monoclonal antibody toolkit for C. elegans. Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs. Berninsone PM, Hirschberg CB Glycoconj. J. 19 (4-5) :325-30 [PMID : 12975612] The nematode Caenorhabditis elegans as a model to study the roles of proteoglycans. |
Functional Descriptions |
sqv-8 The sqv-8 gene encodes a glucuronyl transferase, biochemically active in vitro, that is required for cytokinesis of one-cell embryos and for vulval morphogenesis; SQV-8 is homologous to three distinct glucuronyl transferases (GlcAT-I, GlcAT-P and GlcAT-D) that play a role in the synthesis of different glycoconjugates; the common requirement for SQV-8 in both cytokinesis and morphogenesis may be to promote filling an extracellular space with hygroscopic proteoglycans (either in the eggshell, or underneath the L4 cuticle), which in turn may cause the space to fill with fluid. |
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Deletion Mutants | mn63 n2822 n2825 n2843 n2847 n2850 n2851 n3036 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Expression |
Expression
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WBGene00005026 Phenotype Homology_paralog |
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Phenotype | Definition | RNAi / Allele | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
bloated
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"Worms appears turgid or swollen as a result of an excess number of eggs being retained in the uterus." [WB:WBPerson2021, WB:WBPerson557] |
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comma arrest emb
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"Cessation of development during the middle stage in embryogenesis of the worm in which the embryo is slightly folded within the eggshell (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557] |
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hermaphrodite fertility reduced
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"Hermaphrodites exhibit a reduction in the production of new individuals owing to defects in gamete competence and/or function compared to control animals." [WB:WBPerson2021, WB:WBPerson557] |
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hermaphrodite self sterile
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"Hermaphrodites fail to produce any viable progeny via self-fertilization. However, such hermaphrodites can successfully reproduce if mated to control males." [WB:WBPaper00001075, WB:WBPerson2021] |
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hermaphrodite sterile
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"Hermaphrodites fail to generate viable self-progeny and/or cross-progeny." [WB:WBPerson2021, WB:WBPerson557] |
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larval arrest
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"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021] |
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late embryonic arrest
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"Cessation of development during the final stages of embryogenesis. In C. elegans, this stage normally occurs 620-800 min after the first cleavage at 20C and is the stage after elongation." [WB:WBPerson101] |
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lethal
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"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021] |
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lumpy
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"Animals have protrusions or bumps on their exterior which are not characteristic of control animals." [WB:WBPerson557] |
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one cell arrest early emb
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"Embryos fail to divide and arrest as one-cell embryos." [WB:cab, WB:cgc5599] |
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oocyte morphology variant
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"Haploid female germ cells (oocytes) exhibit variations in their overall structure, appearance or contents compared to control." [WB:WBPaper00001883, WB:WBPerson2021] |
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pharyngeal development variant
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"Variations in the progression of the pharynx (feeding organ) over time, from its formation to the mature structure compared to control. In C. elegans the pharynx is divided into the anterior and posterior regions. The anterior region includes the corpus (procorpus and metacorpus - first bulb) and the posterior region includes the isthmus and terminal bulb (second bulb)." [GO:0043282, WB:WBPerson2021] |
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squashed vulva
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"The apparent partial collapse of the vulval invagination and the elongation of central vulva cells often leading to a flattened appearance." [WB:WBPaper00003405] |
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sterile
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"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712] |
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