Smith degradation, which consists of partial periodate oxidation and aldehyde-reduction, was developed by Smith et al. for linkage analysis of carbohydrates. 1) 2) The periodate oxidation cleaves between diol. The method was improved to high sensitivity with fluorescence-tagged carbohydrates by Omichi and Hase. 3) |
| Category | Isolation & structural analysis of glycans |
| Protocol Name | Chemical degradation ~Smith degradation |
Authors
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Natsuka, Shunji
*
Department of Biology, Faculty of Science, Niigata University
Nakakita, Shin-ichi
Department of Functional Glycomics, Life Science Research Center, Kagawa University
*To whom correspondence should be addressed.
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| KeyWords |
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Reagents
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sodium metaperiodate (Wako Pure Chemical Industries Ltd., Osaka, Japan) |
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sodium acetate (Wako Pure Chemical Industries Ltd.) |
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sodium borohydride (Wako Pure Chemical Industries Ltd.) |
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acetic acid (Wako Pure Chemical Industries Ltd.) |
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ammonia solution (Wako Pure Chemical Industries Ltd.) |
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| Methods |
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1. |
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| 1) |
Partially oxidize a pyridylamino (PA-) sugar chain (0.01–1 nmol) with a mixture of 20 μL of 50 mM sodium metaperiodate solution and 20 μL of 0.1 M sodium acetate, pH 4.0 at 0°C for 15 min in the dark. |
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| 2) |
Add 40 μL of 0.26 M sodium borohydride solution. |
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| 4) |
Add 10 μL of acetic acid and mix well. |
Comment 0
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| 5) |
Neutralize with aqueous ammonia solution. |
Comment 0
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| Initial amount | |
| Copyrights |
 Attribution-Non-Commercial Share Alike
This work is released underCreative Commons licenses
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| Date of registration：2014-07-30 11:29:45 |
- Abdel-Akher, M., Hamilton, J.K., Montgomery, R., and Smith, F. (1952) A new procedure for the determination of the fine structure of polysaccharides. J. Am. Chem. Soc. 74, 4970–4971 [PMID : not found]
- Smith, F., and Van Cleve, J.W. (1955) Reduction of the products of periodate oxidation of carbohydrates. I. Hydrogenation with raney nickel of te dialdehydes from the methyl glycopyranosides. J. Am. Chem. Soc. 77, 3091–4971 [PMID : not found]
- Omichi, K., and Hase, S. (1995) Determination of the linkage positions of reducing-end residues of oligosaccharides by partial periodate oxidation of pyridylaminated derivatives. Anal. Biochem. 227, 404–407 [PMID : 7573970]
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How to Cite this Work in an article:
Natsuka, Shunji,
Nakakita, Shin-ichi,
(2014). GlycoPOD https://jcggdb.jp/GlycoPOD.
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Natsuka, Shunji,
Nakakita, Shin-ichi,
(2014).
Chemical degradation ~Smith degradation.
Retrieved 26,4,2024 ,
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Nakakita, Shin-ichi,
(2014).
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