JCEG ID JCEG182
Name bus-2      WormBase
Human Ortholog

GGDB:B3GNT5 G26 

OMIM:

GGDB Score E-val
B3GALT1 80 4e-17
B3GNT5 70 4e-14
B3GALT2 67 4e-13
B3GNT4 64 3e-12
B3GNT6 62 1e-11
B3GNT8 61 2e-11
B3GALT5 59 1e-10
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name b3GalT1/b3GnT orthologs
Locus Tag K08D12.5
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
gg_motif:M1,M3,M4,M6
Sequence CDS
Protein
References

Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Yook K, Hodgkin J Genetics (2006) 175 (2) :681-97 [PMID : 17151260] Mos1 mutagenesis reveals a diversity of mechanisms affecting response of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms.

Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans.

Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans.

Palaima E, Leymarie N, Stroud D, Mizanur RM, Hodgkin J, Gravato-Nobre MJ, Costello CE, Cipollo JF J. Biol. Chem. (2010) 285 (23) :17662-72 [PMID : 20385555] The Caenorhabditis elegans bus-2 mutant reveals a new class of O-glycans affecting bacterial resistance.

WormBase (WS254)

Functional Descriptions bus-2

bus-2 encodes a predicted glycosyltransferase that contains conserved domains of core-1 beta1,3 galactosyltransferases; bus-2 is required for bacterial infection, and for fully normal cuticle integrity; specifically, bus-2 is needed both for colonization by Microbacterium nematophilum (and the tail-swelling response to M. nematophilum infection), and for attachment of Yersinia pseudotuberculosis biofilms; bus-2 mutant animals exhibit defects in core-1 O-glycan synthesis, while also showing a concomitant increase in novel fucosyl O-glycans; a bus-2::RFP reporter is expressed in the posterior intestine as well as the hypodermal seam cells and spermatheca.
Deletion Mutants
In Situ clone=163c10   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005319

spermatheca "an accordion-like tube that contains sperm and is the site of oocyte fertilization." [WBPaper:00038216] Certain WB:Expr9274 WBPaper00037686 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] Certain WB:Expr9274 WBPaper00037686 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Partial WB:Expr9274 WBPaper00037686 WormAtlas
WB WBbt:0006761

head muscle "body wall muscle in the head, including first eight cells of each muscle quadrants which are innervated by nerve ring neurons." [WB:Paper00001594, WB:rynl] Certain WB:Expr9274 WBPaper00037686

WBGene00044618
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
bacterially unswollen

"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144907   WBPaper00026735   Observed
Allele: WBVar00144908   WBPaper00026735   Observed
Allele: WBVar00144918   WBPaper00026735   Observed
Allele: WBVar00144936   WBPaper00026735   Observed
Allele: WBVar00144990   WBPaper00026735   Observed
Allele: WBVar00144994   WBPaper00026735   Observed
Allele: WBVar00144995   WBPaper00026735   Observed
biofilm absent head

"Animals can move thorough a lawn of bacterially produced biofilm without accumulating an enormous amount of biofilm on its nose. When C. elegans is exposed to certain bacteria (e.g., Y. pestis), a biofilm accumulates on a worm's head. The presence of this biofilm inhibits feeding by the worm, and thereby prevents growth." [WB:cab, WB:WBPaper00005238]
Allele: WBVar00144918   WBPaper00029153   Observed
cuticle fragile

"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
Allele: WBVar00144918   WBPaper00026735   Observed
organism pathogen response variant

"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
Allele: WBVar00144918   WBPaper00037686   Observed
pathogen adherence defect

"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021]
Allele: WBVar00144918   WBPaper00026735   Observed
pathogen resistance increased

"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBPerson261   WBVar00144918   Observed
post hermaphrodite contact variant

"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557]
Allele: WBVar00144918   WBPaper00037686   Observed


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