JCEG ID |
JCEG182 |
Name |
bus-2
WormBase
|
Human Ortholog |
GGDB:B3GNT5 G26
OMIM:
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
|
Family Name |
b3GalT1/b3GnT orthologs |
Locus Tag |
K08D12.5
|
RNAi phenotype |
phenotype
|
WT
|
Microscope observation (Image)
|
|
COPAS (Image)
|
Normal
|
hsp-4::GFP (Image)
|
|
|
Sequence Features |
kr:Detected
tm:Detected
gg_motif:M1,M3,M4,M6
|
Sequence |
CDS
atgatagtttcccgacggattccagttctcgcttacctatttctcagttg cctcatcgccctctacgccttcaaatttcacctctccaccctagttgatg gtattatttttaacaatgaagacgatccaacaccatttgactttgcatta agctttcagttgaagccagagctacccgatgagctcccggctccaaatgt tttagttctcgtgacaactattgcttcggaatttgaaatgagaaatcaag tcagaaaatcatgggctaattatacaagtaatgcagttagagtcaagttt ttgatgggaattccggccgataagcaactcccactgatccagaaagaaag tcaagaattcaatgatttaataatagcggatttagatgaaggctattata gtttagcgtcaaaaaccatggcaatgcttatttataaaactaggtactac ccggacaccaaatgccttgttaaagctgacgtggacaatgttctaattct tcgaaattatgagcgactttgcgaagaagcagttgcccctctaattctgg gaaaatgcgatgtatctagaacggtactacggaataccacaaaatgggcc gtgccagaatttgtgtattccgagccagtttacccgacttattgctccac gggaacttatgtgttggctgggaaaactgtgccgcagagtcttatcaagg aagctatgagtagcccatttgcaaattccttgaacttcagaaaactctcg gaagatgtgatattcacgggaattctcgccgaaaaagccggaataaaacg ccgtcatatcaatgggctctcgtttttcgagattcccgaattcttttgtc gaaatggcttcaaaacgacgtacagtacccatttgttgagtgataagaat cctgtcaagaatttctataaactcatgaaaatcgagggagttctatgcag gtggtgggatccgtatgcggttcggaggtggcgatcttga
atgatagtttcccgacggattccagttctcgcttacctatttctcagttg cctcatcgccctctacgccttcaaatttcacctctccaccctagttgatg aagacgatccaacaccatttgactttgcattaagctttcagttgaagcca gagctacccgatgagctcccggctccaaatgttttagttctcgtgacaac tattgcttcggaatttgaaatgagaaatcaagtcagaaaatcatgggcta attatacaagtaatgcagttagagtcaagtttttgatgggaattccggcc gataagcaactcccactgatccagaaagaaagtcaagaattcaatgattt aataatagcggatttagatgaaggctattatagtttagcgtcaaaaacca tggcaatgcttatttataaaactaggtactacccggacaccaaatgcctt gttaaagctgacgtggacaatgttctaattcttcgaaattatgagcgact ttgcgaagaagcagttgcccctctaattctgggaaaatgcgatgtatcta gaacggtactacggaataccacaaaatgggccgtgccagaatttgtgtat tccgagccagtttacccgacttattgctccacgggaacttatgtgttggc tgggaaaactgtgccgcagagtcttatcaaggaagctatgagtagcccat ttgcaaattccttgaacttcagaaaactctcggaagatgtgatattcacg ggaattctcgccgaaaaagccggaataaaacgccgtcatatcaatgggct ctcgtttttcgagattcccgaattcttttgtcgaaatggcttcaaaacga cgtacagtacccatttgttgagtgataagaatcctgtcaagaatttctat aaactcatgaaaatcgagggagttctatgcaggtggtgggatccgtatgc ggttcggaggtggcgatcttga
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Protein
MIVSRRIPVLAYLFLSCLIALYAFKFHLSTLVDGIIFNNEDDPTPFDFALSFQLKPELPD ELPAPNVLVLVTTIASEFEMRNQVRKSWANYTSNAVRVKFLMGIPADKQLPLIQKESQEF NDLIIADLDEGYYSLASKTMAMLIYKTRYYPDTKCLVKADVDNVLILRNYERLCEEAVAP LILGKCDVSRTVLRNTTKWAVPEFVYSEPVYPTYCSTGTYVLAGKTVPQSLIKEAMSSPF ANSLNFRKLSEDVIFTGILAEKAGIKRRHINGLSFFEIPEFFCRNGFKTTYSTHLLSDKN PVKNFYKLMKIEGVLCRWWDPYAVRRWRS
MIVSRRIPVLAYLFLSCLIALYAFKFHLSTLVDEDDPTPFDFALSFQLKPELPDELPAPN VLVLVTTIASEFEMRNQVRKSWANYTSNAVRVKFLMGIPADKQLPLIQKESQEFNDLIIA DLDEGYYSLASKTMAMLIYKTRYYPDTKCLVKADVDNVLILRNYERLCEEAVAPLILGKC DVSRTVLRNTTKWAVPEFVYSEPVYPTYCSTGTYVLAGKTVPQSLIKEAMSSPFANSLNF RKLSEDVIFTGILAEKAGIKRRHINGLSFFEIPEFFCRNGFKTTYSTHLLSDKNPVKNFY KLMKIEGVLCRWWDPYAVRRWRS
|
References |
Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J
Genetics
(2005)
171
(3)
:1033-45
[PMID : 16079230]
Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.
Yook K, Hodgkin J
Genetics
(2006)
175
(2)
:681-97
[PMID : 17151260]
Mos1 mutagenesis reveals a diversity of mechanisms affecting response of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.
Berninsone PM
WormBook
(2006)
:1-22
[PMID : 18050430]
Carbohydrates and glycosylation.
Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K
Genetics
(2007)
176
(1)
:221-30
[PMID : 17339204]
Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms.
Drace K, Darby C
Appl. Environ. Microbiol.
(2008)
74
(14)
:4509-15
[PMID : 18515487]
The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans.
Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J
Genetics
(2010)
187
(1)
:141-55
[PMID : 20980242]
Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans.
Palaima E, Leymarie N, Stroud D, Mizanur RM, Hodgkin J, Gravato-Nobre MJ, Costello CE, Cipollo JF
J. Biol. Chem.
(2010)
285
(23)
:17662-72
[PMID : 20385555]
The Caenorhabditis elegans bus-2 mutant reveals a new class of O-glycans affecting bacterial resistance.
|
Functional Descriptions |
bus-2
bus-2 encodes a predicted glycosyltransferase that contains conserved domains of core-1 beta1,3 galactosyltransferases; bus-2 is required for bacterial infection, and for fully normal cuticle integrity; specifically, bus-2 is needed both for colonization by Microbacterium nematophilum (and the tail-swelling response to M. nematophilum infection), and for attachment of Yersinia pseudotuberculosis biofilms; bus-2 mutant animals exhibit defects in core-1 O-glycan synthesis, while also showing a concomitant increase in novel fucosyl O-glycans; a bus-2::RFP reporter is expressed in the posterior intestine as well as the hypodermal seam cells and spermatheca.
|
Deletion Mutants |
|
In Situ |
clone=163c10
|
Expression |
Expression
db_name |
wbbt |
name |
def |
reliability |
expr_pattern |
paper |
WormAtlas |
WB |
WBbt:0005319
|
spermatheca |
"an accordion-like tube that contains sperm and is the site of oocyte fertilization." [WBPaper:00038216] |
Certain |
WB:Expr9274
|
WBPaper00037686
|
WormAtlas
|
WB |
WBbt:0005753
|
seam cell |
"a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] |
Certain |
WB:Expr9274
|
WBPaper00037686
|
WormAtlas
|
WB |
WBbt:0005772
|
intestine |
"A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] |
Partial |
WB:Expr9274
|
WBPaper00037686
|
WormAtlas
|
WB |
WBbt:0006761
|
head muscle |
"body wall muscle in the head, including first eight cells of each muscle quadrants which are innervated by nerve ring neurons." [WB:Paper00001594, WB:rynl] |
Certain |
WB:Expr9274
|
WBPaper00037686
|
|
|
WBGene00044618
Phenotype
Homology_paralog
|
|
Phenotype |
Definition |
RNAi / Allele |
bacterially unswollen
|
"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735]
|
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biofilm absent head
|
"Animals can move thorough a lawn of bacterially produced biofilm without accumulating an enormous amount of biofilm on its nose. When C. elegans is exposed to certain bacteria (e.g., Y. pestis), a biofilm accumulates on a worm's head. The presence of this biofilm inhibits feeding by the worm, and thereby prevents growth." [WB:cab, WB:WBPaper00005238]
|
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cuticle carbohydrate surface variant
|
"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712]
|
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cuticle fragile
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"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
|
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embryonic lethal
|
"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
|
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fertility variant
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"Animals exhibit variations in the time of onset or duration of the fertile period, or production of new individuals that contain some portion of their genetic material inherited from that organism compared to control." [GO:0000003, WB:WBPerson2021, WB:WBPerson557]
|
|
larval arrest
|
"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
|
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larval lethal
|
"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
|
|
lethal
|
"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021]
|
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maternal sterile
|
" Animals produce no or very few embryos as a result of experimental treatment. This is in contrast to sterility due to genetic perturbations (maternal effect sterility)." [WB:cab, WB:cgc5599, WB:cgc7141, WB:WBPerson557]
|
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organism morphology variant
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"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395]
|
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organism pathogen response variant
|
"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
|
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pathogen adherence defect
|
"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021]
|
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pathogen resistance increased
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"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
|
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post hermaphrodite contact variant
|
"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557]
|
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postembryonic development variant
|
"Any variation in the progression of an animal over time, from hatching to adulthood, compared to control." [WB:WBPerson2021]
|
|
skiddy
|
"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735]
|
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slow growth
|
"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
|
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sterile progeny
|
"Any variation that compromises the reproductive ability of the progeny of an animal." [WB:WBPerson2021, WB:WBPerson557]
|
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Variant
|
"Animals exhibit variations compared to a given control." [WB:WBPerson557]
|
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