JCEG ID | JCEG166 | |||||||||||||||||||||||||||
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Name | sqv-6 WormBase NCBI Gene | |||||||||||||||||||||||||||
Human Ortholog |
OrthoList : XYLT2 XYLT1 (Ensembl Compara)
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Family Name | XylT | |||||||||||||||||||||||||||
Locus Tag | Y50D4C.4 | |||||||||||||||||||||||||||
RNAi phenotype |
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Sequence Features |
kr: soluble |
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Sequence | CDS |
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Protein |
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References |
Herman T, Horvitz HR Cold Spring Harb. Symp. Quant. Biol. (1997) 62 :353-9 [PMID : 9598369] Mutations that perturb vulval invagination in C. elegans. Herman T, Hartwieg E, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (1999) 96 (3) :968-73 [PMID : 9927677] sqv mutants of Caenorhabditis elegans are defective in vulval epithelial invagination. Hwang HY, Olson SK, Brown JR, Esko JD, Horvitz HR J. Biol. Chem. (2003) 278 (14) :11735-8 [PMID : 12584198] The Caenorhabditis elegans genes sqv-2 and sqv-6, which are required for vulval morphogenesis, encode glycosaminoglycan galactosyltransferase II and xylosyltransferase. Sugahara K, Mikami T, Uyama T, Mizuguchi S, Nomura K, Kitagawa H Curr. Opin. Struct. Biol. (2003) 13 (5) :612-20 [PMID : 14568617] Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate. Wang H, Spang A, Sullivan MA, Hryhorenko J, Hagen FK Mol. Biol. Cell (2005) 16 (9) :4202-13 [PMID : 15987734] The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation. Sternberg PW WormBook (2005) :1-28 [PMID : 18050418] Vulval development. Brunner A, Kolarich D, Voglmeir J, Paschinger K, Wilson IB Glycoconj. J. (2006) 23 (7-8) :543-54 [PMID : 17006645] Comparative characterisation of recombinant invertebrate and vertebrate peptide O-Xylosyltransferases. Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation. Franks DM, Izumikawa T, Kitagawa H, Sugahara K, Okkema PG Dev. Biol. (2006) 296 (2) :409-20 [PMID : 16828468] C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans. Updike DL, Strome S Genetics (2009) 183 (4) :1397-419 [PMID : 19805813] A genomewide RNAi screen for genes that affect the stability, distribution and function of P granules in Caenorhabditis elegans. Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs. Berninsone PM, Hirschberg CB Glycoconj. J. 19 (4-5) :325-30 [PMID : 12975612] The nematode Caenorhabditis elegans as a model to study the roles of proteoglycans. |
Functional Descriptions |
sqv-6 sqv-6 encodes a xylosyltransferase, active in cell culture, that is required for cytokinesis of one-cell embryos and for vulval morphogenesis; SQV-6 is predicted to begin the synthesis of glycosaminoglycans by adding xylose to their protein cores; the common requirement for SQV-6 in both cytokinesis and morphogenesis may be to promote filling an extracellular space with hygroscopic proteoglycans (either in the eggshell, or underneath the L4 cuticle), which in turn may cause the space to fill with fluid. |
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Deletion Mutants | n2845 tm834 | |||
In Situ | ||||
Expression | Expression | |||
WBGene00005024 Phenotype Homology_paralog |
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Phenotype | Definition | RNAi / Allele | ||
bloated
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"Worms appears turgid or swollen as a result of an excess number of eggs being retained in the uterus." [WB:WBPerson2021, WB:WBPerson557] |
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cytokinesis variant emb
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"Any variation that disrupts the cytoplasmic division of a cell during embryonic development compared to control." [GO:0000910, WB:WBPerson2021] |
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egg laying defective
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"Eggs are not laid, laid at a slower rate or eggs are laid at a later stage of development compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPaper00025054] |
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hermaphrodite self sterile
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"Hermaphrodites fail to produce any viable progeny via self-fertilization. However, such hermaphrodites can successfully reproduce if mated to control males." [WB:WBPaper00001075, WB:WBPerson2021] |
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hermaphrodite sterile
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"Hermaphrodites fail to generate viable self-progeny and/or cross-progeny." [WB:WBPerson2021, WB:WBPerson557] |
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life span variant
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"Adult life span is either longer or shorter than typical of control animals." [GO:0008340, WB:cab, WB:WBPaper00005863, WB:WBPaper00026717, WB:WBPerson557] |
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locomotion variant
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"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712] |
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multiple nuclei early emb
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"Embryos contain more than one nucleus per cell in the inviable one- to four-cell embryo." [WB:cab, WB:cgc5599] |
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neuron cell body absent misplaced
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"The body of a neuron is not where it should be when compared to control animals; it is absent or misplaced." [WB:WBPaper00000502, WB:WBPaper00000635, WB:WBPerson712] |
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one cell arrest early emb
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"Embryos fail to divide and arrest as one-cell embryos." [WB:cab, WB:cgc5599] |
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oocyte morphology variant
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"Haploid female germ cells (oocytes) exhibit variations in their overall structure, appearance or contents compared to control." [WB:WBPaper00001883, WB:WBPerson2021] |
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reduced brood size
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"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557] |
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small
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"Animals are shorter and thinner than control animals at the same developmental stage." [WB:cab, WB:cgc31] |
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squashed vulva
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"The apparent partial collapse of the vulval invagination and the elongation of central vulva cells often leading to a flattened appearance." [WB:WBPaper00003405] |
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vulval invagination variant L4
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"The characteristic Christmas-tree shaped invagination formed by the terminal vulval cells at the mid-late L4 stage is altered." [WB:WBPaper00001738, WB:WBPerson2021] |
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