JCEG ID JCEG141
Name ostd-1      WormBase
Human Ortholog

GGDB:RPN2 

OMIM: 180490

OrthoList :   RPN2  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
MAPPED 0.000006 19668339
Family Name Oligosaccharyltransferase complex
Locus Tag M01A10.3
RNAi phenotype
phenotype
Microscope observation (Image)
COPAS (Image)
hsp-4::GFP (Image)
Sequence Features
Sequence CDS
Protein
References

Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J Nature (2000) 408 (6810) :325-30 [PMID : 11099033] Functional genomic analysis of C. elegans chromosome I by systematic RNA interference.

Zipperlen P, Fraser AG, Kamath RS, Martinez-Campos M, Ahringer J EMBO J. (2001) 20 (15) :3984-92 [PMID : 11483502] Roles for 147 embryonic lethal genes on C.elegans chromosome I identified by RNA interference and video microscopy.

Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.

Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ Nature (2005) 434 (7032) :462-9 [PMID : 15791247] Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

Wang H, Spang A, Sullivan MA, Hryhorenko J, Hagen FK Mol. Biol. Cell (2005) 16 (9) :4202-13 [PMID : 15987734] The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Balklava Z, Pant S, Fares H, Grant BD Nat. Cell Biol. (2007) 9 (9) :1066-73 [PMID : 17704769] Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K Cell (2011) 145 (3) :470-82 [PMID : 21529718] A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.

Winter JF, Hopfner S, Korn K, Farnung BO, Bradshaw CR, Marsico G, Volkmer M, Habermann B, Zerial M Nat. Cell Biol. (2012) 14 (7) :666-76 [PMID : 22634595] Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity.

[PMID : 24130834]

WormBase (WS254)

Functional Descriptions ostd-1

Deletion Mutants
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003681

pharynx "the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine." [WB:rynl] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0005300

ventral cord neuron "neuron with cell body associated with the ventral nerve cord." [WB:Paper00000938] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0005735

nervous system "Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal's internal and external environments and to initiate and integrate its effector responses and activities." [ISBN:0-14-051288-8, WB:rynl] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0005747

reproductive system WB:Expr6399 WBPaper00006525
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0005813

body wall musculature "Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion." [ISBN:0-87969-433-5] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0005821

vulval muscle "muscle associated with hermaphrodite vulva." [WB:rynl] WB:Expr6399 WBPaper00006525 WormAtlas
WB WBbt:0006751

head neuron "neuron with its cell body situated in the head, excluding the pharynx." [WB:rynl] WB:Expr6399 WBPaper00006525
WB WBbt:0006759

tail neuron "neuron with its cell body situated in the tail, posterior to rectum." [WB:rynl] WB:Expr6399 WBPaper00006525

WBGene00019693
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
chromosome condensation variant

"Any variation in the progressive compaction of dispersed interphase chromatin into threadlike chromosomes prior to mitotic or meiotic nuclear division, or during apoptosis, in eukaryotic cells compared to control." [GO:0030261, WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
cytokinesis variant emb

"Any variation that disrupts the cytoplasmic division of a cell during embryonic development compared to control." [GO:0000910, WB:WBPerson2021]
RNAi: WBRNAi00061134   WBPaper00026630   Observed
diakinesis progression during oogenesis variant

"Developing oocytes show defects in their entry, progression through or exit from the diakinesis stage compared to control animals. Diakinesis is a cell cycle process comprising the steps by which a cell progresses through the final stage of prophase I in meiosis; the transition to meiotic metaphase I." [GO:0000241, WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
embryo osmotic integrity defective early emb

"Variations in the ability of an embryo to maintain osmotic balance. In C.elegans this can result in an embryo filling the egg shell and lysing." [WB:cab, WB:cgc7141]
RNAi: WBRNAi00050714   WBPaper00025054   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00000083   WBPaper00004402   Observed
RNAi: WBRNAi00025963   WBPaper00006395   Observed
RNAi: WBRNAi00025964   WBPaper00006395   Observed
RNAi: WBRNAi00050714   WBPaper00025054   Observed
excess maternal pronucleus early emb

"One-cell embryos have two or more maternal pronuclei." [WB:cab, WB:cgc5599]
RNAi: WBRNAi00081356   WBPaper00004811   Observed
foraging behavior variant

"Variations in the behavior by which an organism locates food compared to control animals. In C. elegans roaming or dwelling activity on food are two examples of parameters used to assess this behavior. Roaming is defined as moving in a high-speed forward direction with brief backward movement, often traversing wide regions. Dwelling is defined as low-speed/high turning movement restricted to a confined region." [GO:0060756, WB:WBPaper00005621, WB:WBPaper00031455, WB:WBPerson712]
RNAi: WBRNAi00105932   WBPaper00047025   Observed
RNAi: WBRNAi00106007   WBPaper00047025   Observed
germ cell compartment anucleate

"Germ cell compartments do not contain a nucleus." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell compartment expansion variant

"Animals exhibit variations in the increase in size of compartments of the germline. In the C. elegans germline, syncytial compartments enlarge just prior to and coincident with the loop region, eventually abscising, to become fully enclosed germ cells (oocytes)." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell compartment morphology variant

"Any variation in the form or composition of the compartments in the germline of females/hermaphrodites compared to control animals. In C. elegans these compartments contain a nucleus and are connected to a common cytoplasm (rachis). These compartments eventually undergo cellularization and become oocytes." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell compartment multinucleate

"Germ cell compartments contain multiple nuclei." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell compartment size variant

"Any variation in germ cell compartment size compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell partition absent

"Animals lack germ cell partitions (germ cell compartments)." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germ cell partition morphology variant

"Any variation in the form or composition of the compartment partitions in the germline of females/hermaphrodites that exist when nuclei are still connected to a common cytoplasm (rachis), compared to control animals. " [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
germline nuclear positioning variant

"Variations in the directed movement/or positioning of a nucleus to a specific location within the germline compartment compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
larval arrest

"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
RNAi: WBRNAi00025963   WBPaper00006395   Observed
RNAi: WBRNAi00025964   WBPaper00006395   Observed
RNAi: WBRNAi00106503   WBPaper00047025   Observed
larval lethal

"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
RNAi: WBRNAi00000083   WBPaper00004402   Observed
RNAi: WBRNAi00025963   WBPaper00006395   Observed
locomotion variant

"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712]
RNAi: WBRNAi00000083   WBPaper00004402   Observed
RNAi: WBRNAi00025963   WBPaper00006395   Observed
RNAi: WBRNAi00025964   WBPaper00006395   Observed
multiple nuclei early emb

"Embryos contain more than one nucleus per cell in the inviable one- to four-cell embryo." [WB:cab, WB:cgc5599]
RNAi: WBRNAi00061134   WBPaper00026630   Observed
multiple nuclei oocyte

"Haploid female germ cells (oocytes) contain more than one nucleus." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
nuclear fallout

"Nuclei have fallen out of developing germ cell compartments into the rachis." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
oocyte septum formation variant

"Animals exhibit variations in the partitioning of oocytes from each other compared to control animals." [WB:WBPaper00028947]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
oocytes lack nucleus

"Haploid female germ cells (oocytes) lack a nucleus." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
pattern of transgene expression variant

"Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00070986   WBPaper00030951   Observed
RAB-11 recycling endosome localization variant

"Any variation in the subcellular localization of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane." [WB:WBPaper00041129, WB:WBPerson2987]
RNAi: WBRNAi00090786   WBPaper00041129   Observed
RAB-11 recycling endosome morphology variant

"Any variation in the form or composition of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane." [WB:WBPaper00041129, WB:WBPerson2987]
RNAi: WBRNAi00090786   WBPaper00041129   Observed
rachis wide

"The width of the nucleus-free core of germline cytoplasm in the gonad is larger compared to control animals." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
receptor mediated endocytosis defective

"Animals exhibit defects in the cell-mediated uptake of external materials via receptor activity. Receptor-mediated endocytosis ensures specificity of transport and generally occurs via clathrin-coated pits and vesicles. In C. elegans, this is often judged by the lack of yolk uptake in oocytes." [GO:0006898, pmid:16042554, WB:cab, WB:WBPerson2021]
RNAi: WBRNAi00070986   WBPaper00030951   Observed
sterile

"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
RNAi: WBRNAi00085388   WBPaper00038381   Observed
transgene subcellular localization variant

"Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00090786   WBPaper00041129   Observed
RNAi: WBRNAi00106154   WBPaper00047025   Observed


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