JCEG ID JCEG138
Name ZK686.3      WormBase    NCBI Gene
Human Ortholog

GGDB:MAGT1 TUSC3 

OMIM: 300715 300853 601385 611093

OrthoList :   MAGT1   TUSC3  (Ensembl Compara)

Family Name Oligosaccharyltransferase complex
Locus Tag ZK686.3
RNAi phenotype
phenotype
Microscope observation (Image)
COPAS (Image)
hsp-4::GFP (Image)
Sequence Features
Sequence CDS
Protein
References

Gonczy P, Echeverri C, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA Nature (2000) 408 (6810) :331-6 [PMID : 11099034] Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III.

Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, Welchman DP, Zipperlen P, Ahringer J Nature (2003) 421 (6920) :231-7 [PMID : 12529635] Systematic functional analysis of the Caenorhabditis elegans genome using RNAi.

Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.

Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute J, Orkin SH, Hill DE, van den Heuvel S, Vidal M Genome Res. (2004) 14 (10B) :2162-8 [PMID : 15489339] Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library.

Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ Nature (2005) 434 (7032) :462-9 [PMID : 15791247] Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

Frand AR, Russel S, Ruvkun G PLoS Biol. (2005) 3 (10) :e312 [PMID : 16122351] Functional genomic analysis of C. elegans molting.

Ceron J, Rual JF, Chandra A, Dupuy D, Vidal M, van den Heuvel S BMC Dev. Biol. (2007) 7 :30 [PMID : 17417969] Large-scale RNAi screens identify novel genes that interact with the C. elegans retinoblastoma pathway as well as splicing-related components with synMuv B activity.

Balklava Z, Pant S, Fares H, Grant BD Nat. Cell Biol. (2007) 9 (9) :1066-73 [PMID : 17704769] Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic.

Hamamichi S, Rivas RN, Knight AL, Cao S, Caldwell KA, Caldwell GA Proc. Natl. Acad. Sci. U.S.A. (2008) 105 (2) :728-33 [PMID : 18182484] Hypothesis-based RNAi screening identifies neuroprotective genes in a Parkinson's disease model.

Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K Cell (2011) 145 (3) :470-82 [PMID : 21529718] A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.

WormBase (WS254)

Functional Descriptions ZK686.3

ZK686.3 is orthologous to the putative tumor suppressor candidate 3 (TUSC3); TUSC3 is involved in plasma membrane magnesium ion transport; ZK686.3 is also orthologous to the S. cerevisiae dolichyl-diphosphooligosaccharide-protein glycotransferase gamma chain (34-kD) subunit, OST3.
Deletion Mutants
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00026980 : intestine_enriched
 WBPaper00026980 WormAtlas

WBGene00022793
Phenotype
Homology_paralog
Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
accumulated germline cell corpses

"Animals exhibit an increase in the quantity of germ line cells that have undergone programmed cell death but have not been engulfed." [WB:WBPerson712]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
apoptosis variant

"Animals exhibit variations in any processes that influence the programmed cell death pathway. Induction of the apoptotic pathway triggers the activity of proteolytic caspases, which in turn lead to the destruction of the cell." [GO:0006915, WB:WBPerson2021]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
cytoplasmic processing body variant

"Discrete foci within the cytoplasm containing congregations of mRNA translational suppression and degradation mechanisms vary in their arrangement, clustering or are otherwise not well-defined compared to control." [WB:WBPaper00031914, WB:WBPaper00032172, WB:WBPerson712]
RNAi: WBRNAi00094197   WBPaper00040559   Observed
embryo osmotic integrity defective early emb

"Variations in the ability of an embryo to maintain osmotic balance. In C.elegans this can result in an embryo filling the egg shell and lysing." [WB:cab, WB:cgc7141]
RNAi: WBRNAi00059666   WBPaper00025054   Observed
RNAi: WBRNAi00059667   WBPaper00025054   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00002497   WBPaper00004403   Observed
RNAi: WBRNAi00002799   WBPaper00004403   Observed
RNAi: WBRNAi00009493   WBPaper00005654   Observed
RNAi: WBRNAi00027096   WBPaper00006395   Observed
RNAi: WBRNAi00038420   WBPaper00024497   Observed
RNAi: WBRNAi00059666   WBPaper00025054   Observed
RNAi: WBRNAi00059667   WBPaper00025054   Observed
RNAi: WBRNAi00072811   WBPaper00029258   Observed
RNAi: WBRNAi00072812   WBPaper00029258   Observed
germ cell compartment size variant

"Any variation in germ cell compartment size compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
germline nuclear positioning variant

"Variations in the directed movement/or positioning of a nucleus to a specific location within the germline compartment compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
larval arrest

"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
RNAi: WBRNAi00027096   WBPaper00006395   Observed
lethal

"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021]
RNAi: WBRNAi00072810   WBPaper00029258   Observed
RNAi: WBRNAi00072812   WBPaper00029258   Observed
RNAi: WBRNAi00075184   WBPaper00029258   Observed
RNAi: WBRNAi00075185   WBPaper00029258   Observed
RNAi: WBRNAi00075186   WBPaper00029258   Observed
RNAi: WBRNAi00075187   WBPaper00029258   Observed
RNAi: WBRNAi00076897   WBPaper00031384   Observed
molt defect

"Animals exhibit defects in the process of periodic shedding, of part or all of its cuticle, during cuticle replacement that punctuates each larval stage." [GO:0018996, WB:WBPerson712]
RNAi: WBRNAi00066830   WBPaper00026763   Observed
oocyte morphology variant

"Haploid female germ cells (oocytes) exhibit variations in their overall structure, appearance or contents compared to control." [WB:WBPaper00001883, WB:WBPerson2021]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
oocyte septum formation variant

"Animals exhibit variations in the partitioning of oocytes from each other compared to control animals." [WB:WBPaper00028947]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
oogenesis variant

"Any aberrancy in the processes that influence the generation and maturation of oocytes compared to control animals." [GO:0048477, WB:WBPerson2021]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
pattern of transgene expression variant

"Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00071318   WBPaper00030951   Observed
protruding vulva

"Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva." [WB:WBPaper00004408, WB:WBPerson2021]
RNAi: WBRNAi00075184   WBPaper00029258   Observed
RNAi: WBRNAi00075185   WBPaper00029258   Observed
RNAi: WBRNAi00075186   WBPaper00029258   Observed
RNAi: WBRNAi00075187   WBPaper00029258   Observed
rachis morphology variant

"Any variation in the form, structure or composition of the nucleus-free core of germ line cytoplasm in the gonad compared to control animals. In the C. elegans germline, nuclei exiting mitosis and entering meiosis are arranged along the cortex of the germline, partially enclosed by cellular membrane, forming a nucleus-free core of germline cytoplasm, called the rachis." [WB:WBPaper00031889, WB:WBPerson557]
RNAi: WBRNAi00085632   WBPaper00038381   Observed
receptor mediated endocytosis defective

"Animals exhibit defects in the cell-mediated uptake of external materials via receptor activity. Receptor-mediated endocytosis ensures specificity of transport and generally occurs via clathrin-coated pits and vesicles. In C. elegans, this is often judged by the lack of yolk uptake in oocytes." [GO:0006898, pmid:16042554, WB:cab, WB:WBPerson2021]
RNAi: WBRNAi00071318   WBPaper00030951   Observed
reduced brood size

"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557]
RNAi: WBRNAi00072812   WBPaper00029258   Observed
sick

"Animals exhibit some combination of abnormal features relating to size, movement, body integrity, pigmentation, viability, fertility etc." [WB:WBPaper00000179, WB:WBPaper00000565, WB:WBPerson712]
RNAi: WBRNAi00072810   WBPaper00029258   Observed
RNAi: WBRNAi00072812   WBPaper00029258   Observed
RNAi: WBRNAi00075187   WBPaper00029258   Observed
RNAi: WBRNAi00075188   WBPaper00029258   Observed
RNAi: WBRNAi00075189   WBPaper00029258   Observed
RNAi: WBRNAi00075190   WBPaper00029258   Observed
RNAi: WBRNAi00075191   WBPaper00029258   Observed
sterile

"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
RNAi: WBRNAi00072810   WBPaper00029258   Observed
RNAi: WBRNAi00075184   WBPaper00029258   Observed
RNAi: WBRNAi00075185   WBPaper00029258   Observed
RNAi: WBRNAi00075186   WBPaper00029258   Observed
RNAi: WBRNAi00075187   WBPaper00029258   Observed
RNAi: WBRNAi00075188   WBPaper00029258   Observed
RNAi: WBRNAi00075189   WBPaper00029258   Observed
RNAi: WBRNAi00075190   WBPaper00029258   Observed
RNAi: WBRNAi00075191   WBPaper00029258   Observed
RNAi: WBRNAi00085632   WBPaper00038381   Observed
RNAi: WBRNAi00094197   WBPaper00040559   Observed
thin

"Animals exhibit a reduced girth compared to a control animal of the same developmental stage." [WB:WBPerson712]
RNAi: WBRNAi00075184   WBPaper00029258   Observed
RNAi: WBRNAi00075185   WBPaper00029258   Observed
RNAi: WBRNAi00075186   WBPaper00029258   Observed
RNAi: WBRNAi00075187   WBPaper00029258   Observed
transgene subcellular localization variant

"Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00094197   WBPaper00040559   Observed


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