JCEG ID JCEG088
Name cgt-1      WormBase    NCBI Gene
Human Ortholog

GGDB:UGCG 

OMIM: 602874

OrthoList :   UGCG  (Ensembl Compara)

GGDB Score E-val
UGCG 308 2e-85
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcT
Locus Tag T06C12.10
RNAi phenotype
phenotype
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features tm:soluble
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Ichikawa S, Hirabayashi Y Trends Cell Biol. (1998) 8 (5) :198-202 [PMID : 9695839] Glucosylceramide synthase and glycosphingolipid synthesis.

Leipelt M, Warnecke D, Zahringer U, Ott C, Muller F, Hube B, Heinz E J. Biol. Chem. (2001) 276 (36) :33621-9 [PMID : 11443131] Glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants, and fungi.

Lochnit G, Bongaarts R, Geyer R Int. J. Parasitol. (2005) 35 (8) :911-23 [PMID : 15885697] Searching new targets for anthelminthic strategies: Interference with glycosphingolipid biosynthesis and phosphorylcholine metabolism affects development of Caenorhabditis elegans.

Vazquez-Manrique RP, Gonzalez-Cabo P, Ortiz-Martin I, Ros S, Baylis HA, Palau F Genomics (2006) 89 (3) :392-401 [PMID : 17174523] The frataxin-encoding operon of Caenorhabditis elegans shows complex structure and regulation.

Marza E, Simonsen KT, Faergeman NJ, Lesa GM J. Cell. Sci. (2009) 122 (Pt 6) :822-33 [PMID : 19240113] Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells.

Zhang H, Abraham N, Khan LA, Hall DH, Fleming JT, Gobel V Nat. Cell Biol. (2011) 13 (10) :1189-201 [PMID : 21926990] Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K Glycobiology (2011) 21 (6) :834-48 [PMID : 21325339] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.

WormBase (WS254)

Functional Descriptions cgt-1

cgt-1 encodes a transmembrane protein that is one of three C. elegans ceramide glucosyltransferases; by homology, CGT-1 is predicted to localize to the Golgi membrane and catalyze the transfer of glucose from UDP-glucose to ceramide to synthesize glucosylceramide, a glycosphingolipid precursor; although cgt-1 mutant worms show no obvious defects, animals doubly mutant for cgt-1 and cgt-3 arrest development in the early L1 larval stage, showing impaired feeding ability; in addition, cgt-3;cgt-1 and cgt-3;cgt-1;cgt-2 mutant animals show reduced glucosylceramide levels; a CGT-1::GFP reporter fusion is expressed in pharyngeal tissues, the intestine, the pharyngeal-intestinal and intestinal-rectal valves, the rectal gland cells, unidentified head cells, and the excretory system; cgt-1 expression in the intestine is sufficient to rescue the defects of cgt-3;cgt-1 mutant animals.
Deletion Mutants ok1045 qa1809 tm999 tm1027
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0004510

virR "Intestino-rectal valve" [ISBN:0-87969-307-X] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0004511

virL "Intestino-rectal valve" [ISBN:0-87969-307-X] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005190

GABAergic neuron "neuron that uses GABA as a vesicular neurotransmitter." [WB:rynl] Enriched WBPaper00037950 : GABAergic-motor-neurons_larva_SelectivelyEnriched
WBPaper00037950
WB WBbt:0005451

pharyngeal muscle cell "type of cells that make up muscle layers in the pharynx." [WB:rynl] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005751

coelomocyte "A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles." [WBPaper:00038216] Enriched WBPaper00037950 : coelomocytes_embryo_SelectivelyEnriched, coelomocytes_embryo_enriched
WBPaper00037950 WormAtlas
WB WBbt:0005758

hermaphrodite-specific "cells or anatomical parts specific to the hermaphrodite sex" [WB:rynl] Enriched WBPaper00048690 : gonad-hermaphrodite-biased
WBPaper00048690
WB WBbt:0005767

pharyngeal-intestinal valve "A group of six equivalent cells forms a tightly constructed 'valve' that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine." [WBPaper:00038216] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005767

pharyngeal-intestinal valve "A group of six equivalent cells forms a tightly constructed 'valve' that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine." [WBPaper:00038216] Certain WB:Expr11537 WBPaper00038174 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00037950 : intestine_embryo_SelectivelyEnriched, intestine_embryo_enriched
WBPaper00037950 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr11537 WBPaper00038174 WormAtlas
WB WBbt:0005775

excretory canal "Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom." [WBPaper:00038216] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005775

excretory canal "Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom." [WBPaper:00038216] Certain WB:Expr11537 WBPaper00038174 WormAtlas
WB WBbt:0005777

excretory duct "The extracellular sinus formed by the excretory duct and pore cells." [WBPaper:00038216] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005778

excretory pore "The outlet of the excretory sinus to the exterior environment through the cuticle, on the ventral side of the head, just behind the nerve ring." [WBPaper:00038216] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005797

rectal valve cell "cell that forms intestinal-rectal valve." [WB:rynl] Certain WB:Expr11537 WBPaper00038174 WormAtlas
WB WBbt:0005799

rectal gland cell Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005812

excretory cell "H-shaped cell associated with the excretory system, largest cell in C. elegans." [WB:rynl] Certain WB:Expr8571 WBPaper00032934 WormAtlas
WB WBbt:0005812

excretory cell "H-shaped cell associated with the excretory system, largest cell in C. elegans." [WB:rynl] Enriched WBPaper00037950 : excretory-cell_larva_SelectivelyEnriched, excretory-cell_larva_enriched
WBPaper00037950 WormAtlas

WBGene00011517
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
L1 lethal

"Animals die at the L1 stage of the life cycle." [WB:WBPerson2021]
RNAi: WBRNAi00078080   WBPaper00032934   Observed
reduced brood size

"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557]
RNAi: WBRNAi00083975   WBPaper00025177   Observed


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