Reaction:JCGG-RAC0001687

JCGG ID		JCGG-RAC0001687
Submitter		The Noguchi Institute
Reaction ID		R-0000-001687
Regist Date		2012/06/21 17:37:20
REACTANT
MOLECULE ID		JCGG-COM0002163 (Reaction Tree)
	Reactant Type	(in CH2Cl2)
	Mol	0.3 mmol
MOLECULE ID		JCGG-COM0002164
	Reactant Type	D-biotin
	Mol	0.6 mmol
MOLECULE ID		JCGG-COM0000946 (Reaction Tree)
	Reactant Type	EDC
	Mol	3 mmol
MOLECULE ID		JCGG-COM0000578
	Reactant Type	HOBt
	Mol	4 mmol
PRODUCT
MOLECULE ID		JCGG-COM0002165 (Reaction Tree)
	Yield	93%
REACTION DETAIL
Reaction Time		45 minutes, 16 hours
Reaction Temp		22 degree C, room temp
Solvent		DMF, DMF/CH2Cl2
Comment		1) +all except 22, 2) +22
		The reactants were mixed at 0 degree in Celsius before stirred at room temperature. (second step)
COMMENT
Keywords: O-glycopeptide, N-glycopeptide, synthesis, selectively deprotected building blocks, fluorenylmethoxycarbonyl group, Fmoc, benzyl esters
There are multiple steps in this reaction.
REFERENCE
Reference Id		REF-0000-000196
	Source	PREPARATIVE CARBOHYDRATE CHEMISTRY
Reference Id		REF-0000-000197
	Source	ISBN 0-8247-9802-3
Reference Id		REF-0000-000199
	Source	H. Kunz and G. Braum, unpublished results; G. Braum, Synthese von Glycopeptiden mit photoaktivierbaren Gruppen im Saccharidteil, Dissertation, Universitat Mainz, Germany, 1991