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Glycan Syntheses |
JCGG ID |
JCGG-RAC0001214 | ||||
Submitter |
The Noguchi Institute | ||||
Reaction ID |
R-0000-001220
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Regist Date |
2012/06/21 16:45:41 | ||||
| REACTANT | |||||
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Reactant Type |
NH3 (liquid) | ||||
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Reactant Type |
Na (solid) | ||||
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Reactant Type |
NaHCO3 (sat. aq.) | ||||
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Reactant Type |
Ac2O | ||||
| PRODUCT | |||||
MOLECULE ID |
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Yield |
81% | ||||
| REACTION DETAIL | |||||
Reaction Time |
10 minutes, 120 minutes, 3 hours, 2 hours | ||||
Reaction Temp |
-78 degree C, -78 degree C, 25 degree C, room temp | ||||
Solvent |
NH3, NH3, none, Ac2O | ||||
Comment |
1) NH3+Na, 2) +41, 3) temperature change, 4) +NaHCO3, Ac2O | ||||
| COMMENT | |||||
| Keywords: prostate specific antigen, PSA, N-linked glycan, peptide backbone, antibody, cancer, amino acid, keyhole limpet hemocyanin, KLH | |||||
| There are four phases in this reaction. | |||||
| REFERENCE | |||||
Reference Id |
REF-0000-000061 | ||||
Issn |
Electronic | ||||
Doi |
10.1021/ja8028137 | ||||
PubMed ID |
18798614 | ||||
Journal Name |
Journal of the American Chemical Society. (2008) 130 (41): 13598-607. | ||||
Article Title |
Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments. | ||||
Author |
Vadim Y, Dudkin; Justin S, Miller; Anna S, Dudkina; Christophe, Antczak; David A, Scheinberg; Samuel J, Danishefsky | ||||
Affiliation |
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA. | ||||
Reference Id |
REF-0000-000062 | ||||
Source |
J. AM. CHEM. SOC. 2008, 130, 13598-13607 | ||||
Doi |
10.1021/ja8028137 | ||||
©2008-2013 Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST)
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