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Glycan Syntheses |
JCGG ID |
JCGG-RAC0001211 | ||||
Submitter |
The Noguchi Institute | ||||
Reaction ID |
R-0000-001217
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Regist Date |
2012/06/21 16:45:28 | ||||
| REACTANT | |||||
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Mol |
0.455 mmol | ||||
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Mol |
0.044 mmol | ||||
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Reactant Type |
(BrC6H4)3NSbCl6 | ||||
Mol |
0.5 mmol (total) | ||||
| PRODUCT | |||||
MOLECULE ID |
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Yield |
19% | ||||
| REACTION DETAIL | |||||
Reaction Time |
40 minutes, 20 hours | ||||
Reaction Temp |
10 degree C, room temp | ||||
Solvent |
dry MeCN, dry MeCN | ||||
Comment |
1) 21+(BrC6H4)3NSbCl6, 7, MS 3A, 2) the second addition of (BrC6H4)3NSbCl6 | ||||
| MS 3A was included in the solvent. | |||||
| The reactants were mixed at 10 degree in Celsius, and allowed to warm slowly to room temperature within the reaction time. (second phase) | |||||
| Before the reaction, 21 and 7 were prepared independently and stirred with MS 3A in MeCN for 1 hour at room temperature. | |||||
| 7 was added dropwise. | |||||
| COMMENT | |||||
| Keywords: prostate specific antigen, PSA, N-linked glycan, peptide backbone, antibody, cancer, amino acid, keyhole limpet hemocyanin, KLH | |||||
| There are two phases in this reaction. | |||||
| ATTENTION: MeOTf appearing in Scheme 10 is not mentioned in the written method. | |||||
| ATTENTION: DTBP appearing in Scheme 10 is not mentioned in the written method. | |||||
| ATTENTION: For solvent, dry MeCN is mentioned in the written method, whereas CH2Cl2 is mentioned in Scheme 10. | |||||
| REFERENCE | |||||
Reference Id |
REF-0000-000061 | ||||
Issn |
Electronic | ||||
Doi |
10.1021/ja8028137 | ||||
PubMed ID |
18798614 | ||||
Journal Name |
Journal of the American Chemical Society. (2008) 130 (41): 13598-607. | ||||
Article Title |
Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments. | ||||
Author |
Vadim Y, Dudkin; Justin S, Miller; Anna S, Dudkina; Christophe, Antczak; David A, Scheinberg; Samuel J, Danishefsky | ||||
Affiliation |
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA. | ||||
Reference Id |
REF-0000-000062 | ||||
Source |
J. AM. CHEM. SOC. 2008, 130, 13598-13607 | ||||
Doi |
10.1021/ja8028137 | ||||
©2008-2013 Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST)
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