Reaction:JCGG-RAC0001189

JCGG ID		JCGG-RAC0001189
Submitter		The Noguchi Institute
Reaction ID		R-0000-001195
Regist Date		2012/06/21 16:44:19
REACTANT
MOLECULE ID		JCGG-COM0001356
	Mol	35.9 micro mole
MOLECULE ID		JCGG-COM0001030
	Reactant Type	HATU
	Mol	71 + 24 micro mole
MOLECULE ID		JCGG-COM0000770
	Reactant Type	DMSO
	Volume	100 + 300 micro L
MOLECULE ID		JCGG-COM0000426
	Reactant Type	DIEA
	Mol	52 + 5.7 + 21 + 18 micro mole
MOLECULE ID		JCGG-COM0001287 (Reaction Tree)
	Mol	12 micro mole
PRODUCT
MOLECULE ID		JCGG-COM0001357 (Reaction Tree)
	Yield	61%(from compound 23)
REACTION DETAIL
Reaction Time		11 hours
Reaction Temp		NOT specified
Solvent		DMSO
Comment		24 was added 4 minutes after the start of the reaction.
		The second addition of DMSO was performed immediately after the first addition of DIEA.
		HATU was added twice. (0 hour and 7 hours)
		DIEA was added four times. (0 hour, 7 hours, 9 hours, and 10.5 hours)
COMMENT
Keywords: prostate specific antigen, PSA, N-linked glycan, peptide backbone, antibody, cancer, amino acid, keyhole limpet hemocyanin, KLH
REFERENCE
Reference Id		REF-0000-000061
	Issn	Electronic
	Doi	10.1021/ja8028137
	PubMed ID	18798614
	Journal Name	Journal of the American Chemical Society. (2008) 130 (41): 13598-607.
	Article Title	Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments.
	Author	Vadim Y, Dudkin; Justin S, Miller; Anna S, Dudkina; Christophe, Antczak; David A, Scheinberg; Samuel J, Danishefsky
	Affiliation	Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Reference Id		REF-0000-000062
	Source	J. AM. CHEM. SOC. 2008, 130, 13598-13607
	Doi	10.1021/ja8028137