JCGGDB TOP Glycan Syntheses

Reaction

JCGG ID
 JCGG-RAC0001187
Submitter
 The Noguchi Institute
Reaction ID
 R-0000-001193
Regist Date
 2012/06/21 16:44:13
REACTANT
MOLECULE ID
 JCGG-COM0001283 (Reaction Tree)
MOLECULE ID
 JCGG-COM0000756
Reactant Type
 NH3 (liquid)
MOLECULE ID
 JCGG-COM0000160
Reactant Type
 Na (solid)
MOLECULE ID
 JCGG-COM0000549
Reactant Type
 NaHCO3 (sat. aq.)
MOLECULE ID
 JCGG-COM0000086
Reactant Type
 Ac2O
PRODUCT
MOLECULE ID
 JCGG-COM0001285 (Reaction Tree)
Yield
 65%
REACTION DETAIL
Reaction Time
 10 minutes, 120 minutes, 3 hours, 2 hours
Reaction Temp
 -78 degree C, -78 degree C, 25 degree C, room temp
Solvent
 NH3, NH3, none, Ac2O
Comment
 1) NH3+Na, 2) +22, 3) temperature change, 4) +NaHCO3, Ac2O
Very few were described regarding this reaction.
COMMENT
Keywords: prostate specific antigen, PSA, N-linked glycan, peptide backbone, antibody, cancer, amino acid, keyhole limpet hemocyanin, KLH
There are four phases in this reaction.
REFERENCE
Reference Id
 REF-0000-000061
Issn
 Electronic
Doi
 10.1021/ja8028137
PubMed ID
 18798614
Journal Name
 Journal of the American Chemical Society. (2008) 130 (41): 13598-607.
Article Title
 Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments.
Author
 Vadim Y, Dudkin; Justin S, Miller; Anna S, Dudkina; Christophe, Antczak; David A, Scheinberg; Samuel J, Danishefsky
Affiliation
 Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Reference Id
 REF-0000-000062
Source
 J. AM. CHEM. SOC. 2008, 130, 13598-13607
Doi
 10.1021/ja8028137
©2008-2013 Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST)