Reaction:JCGG-RAC0001185

JCGG ID		JCGG-RAC0001185
Submitter		The Noguchi Institute
Reaction ID		R-0000-001191
Regist Date		2012/06/21 16:44:06
REACTANT
MOLECULE ID		JCGG-COM0001280 (Reaction Tree)
	Mol	41.5 micro mole
MOLECULE ID		JCGG-COM0001281
	Reactant Type	ethylenediamine
	Mol	29.9 mmol
MOLECULE ID		JCGG-COM0000979
	Reactant Type	pyridine
	Mol	37 mmol
MOLECULE ID		JCGG-COM0000086
	Reactant Type	Ac2O
	Mol	26.5 mmol
MOLECULE ID		JCGG-COM0000530
	Reactant Type	NaOMe (25% by weight in MeOH)
	Mol	437 micro mole
PRODUCT
MOLECULE ID		JCGG-COM0001282 (Reaction Tree)
	Yield	72%
REACTION DETAIL
Reaction Time		40 hours, 17 hours, 45.5 hours
Reaction Temp		90 degree C, room temp, NOT specified
Solvent		PhCH3/nBuOH = 2mL/8mL, pyridine/Ac2O, THF/MeOH = 2mL/5mL
Comment		1) 22a+ethylenediamine, 2) +pyridine, Ac2O, 3) +NaOMe
COMMENT
Keywords: prostate specific antigen, PSA, N-linked glycan, peptide backbone, antibody, cancer, amino acid, keyhole limpet hemocyanin, KLH
There are three phases in this reaction.
REFERENCE
Reference Id		REF-0000-000061
	Issn	Electronic
	Doi	10.1021/ja8028137
	PubMed ID	18798614
	Journal Name	Journal of the American Chemical Society. (2008) 130 (41): 13598-607.
	Article Title	Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments.
	Author	Vadim Y, Dudkin; Justin S, Miller; Anna S, Dudkina; Christophe, Antczak; David A, Scheinberg; Samuel J, Danishefsky
	Affiliation	Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Reference Id		REF-0000-000062
	Source	J. AM. CHEM. SOC. 2008, 130, 13598-13607
	Doi	10.1021/ja8028137