JCEG ID JCEG178
Name bre-5      WormBase    NCBI Gene
Human Ortholog

GGDB:B3GALNT1 B3GALT4 B3GALT5 B3GNT3 B3GNT4 B3GNT6 B3GNT7 

OMIM:

OrthoList :   B3GNT7   B3GNT5   B3GNT3   B3GNT9   B3GNT4   B3GNT6   B3GNT2   B3GNT8  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
MAPPED 0.000006 1.178 21041247
UPSTREAM 0.00000006 0.031 (unit increase) [0.019-0.043] 25387707
UPSTREAM 0.733 0.0000000001 0.3 (unit increase) [0.20-0.40] 23583979
UPSTREAM 0.543 0.0000007 0.108 (unit increase) [0.065-0.151] 23568457
MAPPED 0.43 0.000002 (AA) 4.21 (unit decrease) [2.49-5.93] 23599027
DOWNSTREAM 0.26 0.0000000000001 (CA19-9) 0.072 (U/ml decrease) [0.029-0.115] 23300138
GGDB Score E-val
B3GALT5 91 2e-20
B3GALNT1 91 3e-20
B3GNT3 89 1e-19
B3GNT6 89 1e-19
B3GNT7 87 3e-19
B3GALT1 84 3e-18
B3GNT4 82 1e-17
B3GNT5 82 1e-17
B3GALT4 78 2e-16
B3GNT2 71 3e-14
B3GALT2 63 5e-12
B3GNT8 59 9e-11
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name b3GalT1/b3GnT orthologs
Locus Tag T12G3.8
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features tm:soluble
dxd:Detected
gg_motif:M1,M2,M3
Sequence CDS
Protein
References

Marroquin LD, Elyassnia D, Griffitts JS, Feitelson JS, Aroian RV Genetics (2000) 155 (4) :1693-9 [PMID : 10924467] Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans.

Griffitts JS, Whitacre JL, Stevens DE, Aroian RV Science (2001) 293 (5531) :860-4 [PMID : 11486087] Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme.

Griffitts JS, Huffman DL, Whitacre JL, Barrows BD, Marroquin LD, Muller R, Brown JR, Hennet T, Esko JD, Aroian RV J. Biol. Chem. (2003) 278 (46) :45594-602 [PMID : 12944392] Resistance to a bacterial toxin is mediated by removal of a conserved glycosylation pathway required for toxin-host interactions.

Darby C WormBook (2005) :1-15 [PMID : 18050390] Interactions with microbial pathogens.

Mylonakis E, Aballay A Infect. Immun. (2005) 73 (7) :3833-41 [PMID : 15972468] Worms and flies as genetically tractable animal models to study host-pathogen interactions.

Greenwald I WormBook (2005) :1-16 [PMID : 18050403] LIN-12/Notch signaling in C. elegans.

Katic I, Vallier LG, Greenwald I Genetics (2005) 171 (4) :1605-15 [PMID : 16157663] New positive regulators of lin-12 activity in Caenorhabditis elegans include the BRE-5/Brainiac glycosphingolipid biosynthesis enzyme.

Wang H, Spang A, Sullivan MA, Hryhorenko J, Hagen FK Mol. Biol. Cell (2005) 16 (9) :4202-13 [PMID : 15987734] The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation.

Yochem J, Herman RK WormBook (2005) :1-6 [PMID : 18023118] Genetic mosaics.

Anyanful A, Dolan-Livengood JM, Lewis T, Sheth S, Dezalia MN, Sherman MA, Kalman LV, Benian GM, Kalman D Mol. Microbiol. (2005) 57 (4) :988-1007 [PMID : 16091039] Paralysis and killing of Caenorhabditis elegans by enteropathogenic Escherichia coli requires the bacterial tryptophanase gene.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Barrows BD, Haslam SM, Bischof LJ, Morris HR, Dell A, Aroian RV J. Biol. Chem. (2006) 282 (5) :3302-11 [PMID : 17135259] Resistance to Bacillus thuringiensis toxin in Caenorhabditis elegans from loss of fucose.

Ideo H, Fukushima K, Gengyo-Ando K, Mitani S, Dejima K, Nomura K, Yamashita K J. Biol. Chem. (2009) 284 (39) :26493-501 [PMID : 19635802] A Caenorhabditis elegans glycolipid-binding galectin functions in host defense against bacterial infection.

Butschi A, Titz A, Walti MA, Olieric V, Paschinger K, Nobauer K, Guo X, Seeberger PH, Wilson IB, Aebi M, Hengartner MO, Kunzler M PLoS Pathog. (2010) 6 (1) :e1000717 [PMID : 20062796] Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.

Los FC, Kao CY, Smitham J, McDonald KL, Ha C, Peixoto CA, Aroian RV Cell Host Microbe (2011) 9 (2) :147-57 [PMID : 21320697] RAB-5- and RAB-11-dependent vesicle-trafficking pathways are required for plasma membrane repair after attack by bacterial pore-forming toxin.

Zhang H, Abraham N, Khan LA, Hall DH, Fleming JT, Gobel V Nat. Cell Biol. (2011) 13 (10) :1189-201 [PMID : 21926990] Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

WormBase (WS254)

Functional Descriptions bre-5

The bre-5 gene encodes a putative beta-1,3-galactosyltransferase that is predicted to transfer galactose onto proteins or lipids; BRE-5 is required for Cry5B toxicity.
Deletion Mutants
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00040985 : AIN-2_L4_intestine
WBPaper00040985 WormAtlas
WB WBbt:0005813

body wall musculature "Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion." [ISBN:0-87969-433-5] Enriched WBPaper00040985 : AIN-2_asynchronous_muscle
WBPaper00040985 WormAtlas

WBGene00000270
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
Bacillus thuringiensis toxin resistant

"Animals fail to be affected by toxins produced by Bacillus thuringiensis (Bt toxins) in the same manner as control animals." [GO:0042493, WB:WBPaper00004264, WB:WBPaper00004776, WB:WBPerson557]
Allele: WBVar00275391   WBPaper00034766   Observed
organism toxic chemical response variant

"Animals exhibit variations in their response to a specific toxic chemical compared to that observed in control animals." [WB:WBPerson557]
Allele: WBVar00275391   WBPaper00034766   Observed
pesticide response variant

"Animals exhibit variations in their response to any chemical substance that is used for killing pests, compared to that observed in control animals." [WB:WBPerson557]
RNAi: WBRNAi00063080   WBPaper00004776   Observed
pore forming toxin resistant

"Animals fail to respond to the concentration of pore forming toxins that elicits a response in control animals. Alternatively, animals require increased concentration or longer exposure to this compound to elicit the response compared to control animals." [WB:WBPerson2987, WB:WBPerson557]
Allele: WBVar00275391   WBPaper00004264   Observed


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