JCEG ID JCEG176
Name bus-17      WormBase    NCBI Gene
Human Ortholog

GGDB:B3GALT2 

OMIM:

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
UPSTREAM 0.169 0.0000003 1.09 [1.05-1.13] 24162737
GGDB Score E-val
B3GNT5 86 7e-19
B3GNT6 83 6e-18
B3GALT2 82 1e-17
B3GALT1 80 4e-17
B3GNT4 73 8e-15
B3GNT3 72 2e-14
B3GALT4 70 6e-14
B3GNT7 67 5e-13
B3GNT2 64 3e-12
B3GNT8 56 7e-10
B3GALNT2 45 1e-06
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name b3GalT1/b3GnT orthologs
Locus Tag ZK678.8
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features tm:soluble
dxd:NDD
gg_motif:M1,M2
Sequence CDS
Protein
References

Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Yook K, Hodgkin J Genetics (2006) 175 (2) :681-97 [PMID : 17151260] Mos1 mutagenesis reveals a diversity of mechanisms affecting response of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms.

Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans.

Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans.

WormBase (WS254)

Functional Descriptions bus-17

bus-17 encodes a putative GT13 glycosyltransferase whose homologs include human B3GALT2 and C. elegans BRE-5; BUS-17 is required for bacterial infection, as well as for cuticle integrity, long-term survival, and traction during movement; specifically, bus-17 is needed both for colonization by Microbacterium nematophilum (and the tail-swelling response to M. nematophilum infection) and for attachment of Yersinia pseudotuberculosis biofilms; bus-17 is also needed for normal cuticle antigens; BUS-17 is predicted to have an N-terminal signal sequence and a moderately conserved active site; bus-17 and bus-3 function together in vivo, since bus-17(e2695)/+ sometimes shows nonallelic noncomplementation with bus-3(e2696)/+; bus-17 males are sometimes wild-type if their fathers were wild-type.
Deletion Mutants br2 tm1911
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Enriched WBPaper00037950 : hypodermis_embryo_SelectivelyEnriched, hypodermis_embryo_enriched
 WBPaper00037950

WBGene00044630
Phenotype
Homology_paralog
Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
bacterially unswollen

"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144926   WBPaper00026735   Observed
Allele: WBVar00145005   WBPaper00026735   Observed
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed
biofilm absent head

"Animals can move thorough a lawn of bacterially produced biofilm without accumulating an enormous amount of biofilm on its nose. When C. elegans is exposed to certain bacteria (e.g., Y. pestis), a biofilm accumulates on a worm's head. The presence of this biofilm inhibits feeding by the worm, and thereby prevents growth." [WB:cab, WB:WBPaper00005238]
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed
Allele: WBVar00145005   WBPaper00031925   Observed
bleach hypersensitive

"Animals respond to bleach at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557]
Allele: WBPerson261   WBVar00145005   Observed
Allele: WBPerson712   WBVar00145053   Observed
cuticle carbohydrate surface variant

"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712]
Allele: WBVar00145005   WBPaper00026735   Observed
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed
cuticle fragile

"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
Allele: WBVar00145005   WBPaper00026735   Observed
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed
drug hypersensitive

"Animals respond to a specific drug at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557]
Allele: WBPerson261   WBVar00145005   Observed
locomotion variant

"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712]
Allele: WBPerson712   WBVar00145053   Observed
organism hypersensitive desication

"Animals respond to less severe drought-like conditions or a shorter exposure compared to control animals." [WB:WBPerson2021]
Allele: WBVar00145005   WBPaper00026735   Observed
organism pathogen response variant

"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
Allele: WBVar00145005   WBPaper00037686   Observed
pathogen adherence defect

"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021]
Allele: WBVar00145005   WBPaper00026735   Observed
pathogen resistance increased

"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBPerson261   WBVar00145005   Observed
post hermaphrodite contact variant

"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557]
Allele: WBVar00145005   WBPaper00037686   Observed
skiddy

"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00145005   WBPaper00026735   Observed
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed
slow growth

"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
Allele: WBVar00145005   WBPaper00037686   Observed
small

"Animals are shorter and thinner than control animals at the same developmental stage." [WB:cab, WB:cgc31]
Allele: WBVar00145005   WBPaper00037686   Observed
surface antigen negative

"Cuticles or cuticular extracts do not bind antibodies that are observed to bind the surfaces or cuticular extracts of Bristol N2 animals of the same developmental stage." [WB:WBPaper00001280, WB:WBPerson712]
Allele: WBVar00000539   WBPaper00029153   Observed
Allele: WBVar00000548   WBPaper00029153   Observed


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