| JCEG ID |
JCEG150 |
| Name |
pst-2
WormBase
|
| Human Ortholog |
GGDB:SLC35B3
OMIM:
610845
OrthoList :
SLC35B3
(Ensembl Compara)
GWAS Catalog
| STREAM | RAF | p-value | OR | BETA | CI | Study |
| DOWNSTREAM |
|
0.000008
|
|
|
|
24324551
|
|
Deng X 2013-11-20 PLoS One
Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
|
| DOWNSTREAM |
0.49 |
0.000003
(AA)
|
|
0.17
(unit decrease)
|
[0.092-0.248] |
23870195
|
|
Wojczynski MK 2013-07-19 BMC Med Genet
Genetics of coronary artery calcification among African Americans, a meta-analysis.
|
| DOWNSTREAM |
0.48 |
0.000000000000000003
|
|
0.046
(unit increase)
|
[0.035-0.056] |
25628336
|
|
Hromatka BS 2015-01-26 Hum Mol Genet
Genetic variants associated with motion sickness point to roles for inner ear development, neurological processes and glucose homeostasis.
|
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
|
| Family Name |
PAPS synthase and PAPS transporters |
| Locus Tag |
F54E7.1
|
| RNAi phenotype |
|
phenotype
|
|
|
Microscope observation (Image)
|
|
|
COPAS (Image)
|
|
|
hsp-4::GFP (Image)
|
|
|
| Sequence Features |
|
| Sequence |
CDS
ATGACTGCAGCTCAAATACATTCTGATTGTAGTGTTCATATGCTGCCACGTCATGTCAAAGAGGATGTAG
AACCAATTCATCTGTTAGGGTTTAATATTGCCAGAAAACCAAAATGGCTTCAATTTGTTCTTCTTTCTTT
AGCAATATTTATTTTGTACATAGGATACGGGTATATGCAGGAATTAATATTCAAACTACCTGGAATGAAA
CCATTTGGATGGACGTTAACACTTATTCAATTCCTGATTTATAGCGGATGTGGATATACGGAATGTATTA
TTTGGCATAACACGAAACGAATGATCCCTTGGAGAATCTACGGTGTAATTGCCTTTTTCACAGTAGCAAC
AATGGGACTTTCGAATGCATCCGTTGGATATTTAAATTATCCAACTCAAGTGATTTTCAAGTGCTGCAAG
CTAATTCCAGTGCTCATTGGAGGGATTCTGATCCAAGGAAAACGATATGGATGGATTGATATTGGTGCGG
CGATGTTAATGAGTTTAGGAATTATCATGTTCACATTGGCCGATAATAAAGTATCCCCAAATTTCGATTC
TCGAGGATACATCATGATTTGTGGAGCTCTTCTGGCAGATGCTGTAATTGGAAATATTCAAGAAAAGAAT
ATGAAGAAGTACGGTGGATCTAGTAATGAAATGGTTCTCTACTCATATGGAATTGGCTCAGTGTTCATTT
TCGCATTTGTCGTGCTCTCCGGTGAAGTCTTCAGTGCAATTCCATTTTTCCTGGAGAACTCGTGGAAAAC
ATTCGGCTACGCACTGATCCTCTCATGTCTTGGATATCTCGGTGTCAACGTTGTGCTCACTCATATCAAA
GTGTTTGGAGCACTTGTTGCGGTTACTGTGACAACTCTTCGCAAGGCACTGACTATAATTCTGTCGTTCA
TGCTCTTCTCGAAGCCGTTCACCATCGAATATGTTTATGCGGGATCTGTGGTGATGCTGGCAATTTATTT
GAATTTGTACAGCAAGAACAAGACGTCTTGGGATAATATGATAAGAAGATTCGTGGCGAGAGCAATGGGA
TATCATGATGTATCTGTTGCTAGGAAGGATCCAATGACGGTTTAG
ATGGGACTTTCGAATGCATCCGTTGGATATTTAAATTATCCAACTCAAGTGATTTTCAAGTGCTGCAAGC
TAATTCCAGTGCTCATTGGAGGGATTCTGATCCAAGGAAAACGATATGGATGGATTGATATTGGTGCGGC
GATGTTAATGAGTTTAGGAATTATCATGTTCACATTGGCCGATAATAAAGTATCCCCAAATTTCGATTCT
CGAGGATACATCATGATTTGTGGAGCTCTTCTGGCAGATGCTGTAATTGGAAATATTCAAGAAAAGAATA
TGAAGAAGTACGGTGGATCTAGTAATGAAATGGTTCTCTACTCATATGGAATTGGCTCAGTGTTCATTTT
CGCATTTGTCGTGCTCTCCGGTGAAGTCTTCAGTGCAATTCCATTTTTCCTGGAGAACTCGTGGAAAACA
TTCGGCTACGCACTGATCCTCTCATGTCTTGGATATCTCGGTGTCAACGTTGTGCTCACTCATATCAAAG
TGTTTGGAGCACTTGTTGCGGTTACTGTGACAACTCTTCGCAAGGCACTGACTATAATTCTGTCGTTCAT
GCTCTTCTCGAAGCCGTTCACCATCGAATATGTTTATGCGGGATCTGTGGTGATGCTGGCAATTTATTTG
AATTTGTACAGCAAGAACAAGACGTCTTGGGATAATATGATAAGAAGATTCGTGGCGAGAGCAATGGGAT
ATCATGATGTATCTGTTGCTAGGAAGGATCCAATGACGGTTTAG
|
Protein
MTAAQIHSDCSVHMLPRHVKEDVEPIHLLGFNIARKPKWLQFVLLSLAIFILYIGYGYMQELIFKLPGMK
PFGWTLTLIQFLIYSGCGYTECIIWHNTKRMIPWRIYGVIAFFTVATMGLSNASVGYLNYPTQVIFKCCK
LIPVLIGGILIQGKRYGWIDIGAAMLMSLGIIMFTLADNKVSPNFDSRGYIMICGALLADAVIGNIQEKN
MKKYGGSSNEMVLYSYGIGSVFIFAFVVLSGEVFSAIPFFLENSWKTFGYALILSCLGYLGVNVVLTHIK
VFGALVAVTVTTLRKALTIILSFMLFSKPFTIEYVYAGSVVMLAIYLNLYSKNKTSWDNMIRRFVARAMG
YHDVSVARKDPMTV
MGLSNASVGYLNYPTQVIFKCCKLIPVLIGGILIQGKRYGWIDIGAAMLMSLGIIMFTLADNKVSPNFDS
RGYIMICGALLADAVIGNIQEKNMKKYGGSSNEMVLYSYGIGSVFIFAFVVLSGEVFSAIPFFLENSWKT
FGYALILSCLGYLGVNVVLTHIKVFGALVAVTVTTLRKALTIILSFMLFSKPFTIEYVYAGSVVMLAIYL
NLYSKNKTSWDNMIRRFVARAMGYHDVSVARKDPMTV
|
| References |
Bhattacharya R, Townley RA, Berry KL, Bulow HE
J. Cell. Sci.
(2009)
122
(Pt 24)
:4492-504
[PMID : 19920077]
The PAPS transporter PST-1 is required for heparan sulfation and is essential for viability and neural development in C. elegans.
Dejima K, Murata D, Mizuguchi S, Nomura KH, Izumikawa T, Kitagawa H, Gengyo-Ando K, Yoshina S, Ichimiya T, Nishihara S, Mitani S, Nomura K
J. Biol. Chem.
(2010)
285
(32)
:24717-28
[PMID : 20529843]
Two Golgi-resident 3'-Phosphoadenosine 5'-phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.
Shaye DD, Greenwald I
PLoS ONE
(2011)
6
(5)
:e20085
[PMID : 21647448]
OrthoList: a compendium of C. elegans genes with human orthologs.
|
| Functional Descriptions |
pst-2
pst-2 encodes the C. elegans ortholog of the PAPST2 PAPS (3'-phospho-adenosine-5'- phosphosulfate) transporter; a pst-2::gfp reporter fusion is first expressed in ectodermal cells at the ~350-cell stage of embryogenesis; during larval stages, expression is seen in the intestine and, in the adult stage, in the vulva, with weak expression in the hypodermis; PST-2::GFP shows partial localization to the Golgi; pst-2 can partially complement developmental defects seen in pst-1 mutants, suggesting that pst-2 encodes a functional transporter but that it likely has some non-overlapping functions with pst-1 in vivo.
|
| Deletion Mutants |
|
| In Situ |
|
| Expression |
Expression
| db_name |
wbbt |
name |
def |
reliability |
expr_pattern |
paper |
WormAtlas |
| WB |
WBbt:0005733
|
hypodermis |
"epidermal layer" [WB:pws] |
Certain |
WB:Expr8857
|
WBPaper00035477
|
|
| WB |
WBbt:0005733
|
hypodermis |
"epidermal layer" [WB:pws] |
Enriched |
WBPaper00037950 : hypodermis_larva_SelectivelyEnriched, hypodermis_larva_enriched
|
WBPaper00037950
|
|
| WB |
WBbt:0005772
|
intestine |
"A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] |
Certain |
WB:Expr8857
|
WBPaper00035477
|
WormAtlas
|
| WB |
WBbt:0005772
|
intestine |
"A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] |
Certain |
WB:Expr9062
|
WBPaper00036358
|
WormAtlas
|
| WB |
WBbt:0005788
|
pharyngeal gland cell |
"secretory gland cell of the pharynx" [WB:rynl] |
Certain |
WB:Expr9062
|
WBPaper00036358
|
WormAtlas
|
| WB |
WBbt:0006748
|
vulva |
"female genital." [WB:rynl] |
Certain |
WB:Expr8857
|
WBPaper00035477
|
WormAtlas
|
|
WBGene00018827
Phenotype
Homology_paralog
|
|
|
| Phenotype |
Definition |
RNAi / Allele |
|
asymmetric cell division defective early emb
|
"Symmetric (PAR-like) divisions or excessive posterior displacement (zyg-8 like phenotypes)." [WB:cab, WB:cgc7141]
|
|
|
cell division variant
|
"Any variation that alters the physical partitioning and separation of a cell into daughter cells compared to control." [GO:0051301, WB:WBPerson2021]
|
|
|
cell morphology variant
|
"Any variation in the form or composition of the basic structural and functional unit of all organisms compared to control. Includes the plasma membrane and any external encapsulating structures such as the cell wall and cell envelope." [GO:0005623, WB:WBPerson2021, WB:WBPerson557]
|
|
|
cuticle development variant
|
"Any variation in the progression of the formation of the rigid external coating (which is secreted by the hypodermis, seam and some interfacial epithelial cells) over time compared to control animals (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
|
|
|
cuticle fragile
|
"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
|
|
|
cuticle morphology variant
|
"Any variation in the appearance or structure of the rigid external coating which is secreted by the hypodermis, seam and some interfacial epithelial cells compared to control (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
|
|
|
cytokinesis variant emb
|
"Any variation that disrupts the cytoplasmic division of a cell during embryonic development compared to control." [GO:0000910, WB:WBPerson2021]
|
|
|
distal tip cell migration variant
|
" Variations in the migration pattern of distal tip cells compared to control animals. In C. elegans, DTCs located on the anterior and posterior ends of the gonad primordium typically migrate in a symmetrical U-shaped pattern." [GO:0040039, WB:WBPaper00003598, WB:WBPerson2021]
|
|
|
embryonic development variant
|
"Variations in the progression of an embryo over time, from zygote formation to hatching compared to control." [GO:0009790, WB:WBPerson2021]
|
|
|
embryonic lethal
|
"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
|
|
|
gastrulation variant
|
"Variations in the process by which the germ layers become positioned in an embryo compared to control animals. Gastrulation involves the proper ingression of small groups of cells at various times into the blastocoel space." [WB:WBPaper00027251, WB:WBPerson2021]
|
|
heparan sulfate biosynthesis variant
|
"Animals exhibit variations in the chemical reactions and pathways resulting in the formation of the heparan sulfate proteoglycan compared to control." [WB:WBPaper00029024, WB:WBPaper00031153, WB:WBPerson712]
|
|
|
hypodermal cell elongation defective
|
"During epidermal morphogenesis, hypodermal cells do not properly undergo the characteristic cell shape changes necessary for the embryo to elongate." [WB:WBPaper00003991, WB:WBPerson712]
|
|
|
larval arrest
|
"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
|
|
|
larval lethal
|
"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
|
|
|
lethal
|
"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021]
|
|
|
maternal sterile
|
" Animals produce no or very few embryos as a result of experimental treatment. This is in contrast to sterility due to genetic perturbations (maternal effect sterility)." [WB:cab, WB:cgc5599, WB:cgc7141, WB:WBPerson557]
|
|
|
organism morphology variant
|
"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395]
|
|
|
postembryonic development variant
|
"Any variation in the progression of an animal over time, from hatching to adulthood, compared to control." [WB:WBPerson2021]
|
|
|
reduced brood size
|
"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557]
|
|
|
skiddy
|
"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735]
|
|
|
slow growth
|
"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
|
|
|
sterile
|
"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
|
|
|
sterile progeny
|
"Any variation that compromises the reproductive ability of the progeny of an animal." [WB:WBPerson2021, WB:WBPerson557]
|
|
|
Variant
|
"Animals exhibit variations compared to a given control." [WB:WBPerson557]
|
|
|
ventral enclosure variant
|
"Any variation in the process that regulates the migration of ventral epidermal cells toward the ventral midline (to encase underlying cells in an epithelial monolayer) in developing embryos compared to control." [WB:WBPaper00027244, WB:WBPerson2021]
|
|
|
vulva morphology variant
|
"Animals exhibit variations in the gross structure or organization of the reproductive organ required for depositing eggs and for influencing male mating behavior compared to control." [WB:WBPerson712]
|
|
