JCEG ID JCEG137
Name dad-1      WormBase
Human Ortholog

GGDB:DAD1 

OMIM: 600243

OrthoList :   DAD1  (Ensembl Compara)

Family Name Oligosaccharyltransferase complex
Locus Tag F57B10.10
RNAi phenotype
phenotype small, oocyte morphology variant (89% n=29, see reference), hsp-4::GFP increase
Microscope observation (Image)
COPAS (Image)
hsp-4::GFP (Image)

Increase

Sequence Features
Sequence CDS
Protein
References

Sugimoto A, Hozak RR, Nakashima T, Nishimoto T, Rothman JH EMBO J. (1995) 14 (18) :4434-41 [PMID : 7556086] dad-1, an endogenous programmed cell death suppressor in Caenorhabditis elegans and vertebrates.

Hengartner MO Curr. Opin. Genet. Dev. (1996) 6 (1) :34-8 [PMID : 8791487] Programmed cell death in invertebrates.

Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J Nature (2000) 408 (6810) :325-30 [PMID : 11099033] Functional genomic analysis of C. elegans chromosome I by systematic RNA interference.

Zipperlen P, Fraser AG, Kamath RS, Martinez-Campos M, Ahringer J EMBO J. (2001) 20 (15) :3984-92 [PMID : 11483502] Roles for 147 embryonic lethal genes on C.elegans chromosome I identified by RNA interference and video microscopy.

Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.

Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ Nature (2005) 434 (7032) :462-9 [PMID : 15791247] Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

Conradt B, Xue D WormBook (2005) :1-13 [PMID : 18061982] Programmed cell death.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Balklava Z, Pant S, Fares H, Grant BD Nat. Cell Biol. (2007) 9 (9) :1066-73 [PMID : 17704769] Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K Cell (2011) 145 (3) :470-82 [PMID : 21529718] A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.

Hengartner MO Exp. Gerontol. 32 (4-5) :363-74 [PMID : 9315441] Genetic control of programmed cell death and aging in the nematode Caenorhabditis elegans.

WormBase (WS254)

Functional Descriptions dad-1

dad-1 encodes an ortholog of human DAD1 (OMIM:600243) that inhibits cell death and can functionally complement the hamster protein in cultured cells.
Deletion Mutants
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Enriched WBPaper00037950 : hypodermis_larva_enriched
WBPaper00037950
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00026980 : intestine_enriched
WBPaper00026980 WormAtlas

WBGene00000896
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
embryo osmotic integrity defective early emb

"Variations in the ability of an embryo to maintain osmotic balance. In C.elegans this can result in an embryo filling the egg shell and lysing." [WB:cab, WB:cgc7141]
RNAi: WBRNAi00048814   WBPaper00025054   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00000071   WBPaper00004402   Observed
RNAi: WBRNAi00025721   WBPaper00006395   Observed
RNAi: WBRNAi00025722   WBPaper00006395   Observed
RNAi: WBRNAi00048814   WBPaper00025054   Observed
excess maternal pronucleus early emb

"One-cell embryos have two or more maternal pronuclei." [WB:cab, WB:cgc5599]
RNAi: WBRNAi00081357   WBPaper00004811   Observed
germ cell compartment expansion variant

"Animals exhibit variations in the increase in size of compartments of the germline. In the C. elegans germline, syncytial compartments enlarge just prior to and coincident with the loop region, eventually abscising, to become fully enclosed germ cells (oocytes)." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
germ cell compartment morphology variant

"Any variation in the form or composition of the compartments in the germline of females/hermaphrodites compared to control animals. In C. elegans these compartments contain a nucleus and are connected to a common cytoplasm (rachis). These compartments eventually undergo cellularization and become oocytes." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
germ cell compartment multinucleate

"Germ cell compartments contain multiple nuclei." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
germline nuclear positioning variant

"Variations in the directed movement/or positioning of a nucleus to a specific location within the germline compartment compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
larval arrest

"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
RNAi: WBRNAi00025721   WBPaper00006395   Observed
RNAi: WBRNAi00025722   WBPaper00006395   Observed
larval lethal

"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
RNAi: WBRNAi00000071   WBPaper00004402   Observed
locomotion variant

"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712]
RNAi: WBRNAi00025721   WBPaper00006395   Observed
oocyte number decreased

"A decreased number of celullarized haploid female germ cells (oocytes) are present in the gonad compared to control." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
oogenesis variant

"Any aberrancy in the processes that influence the generation and maturation of oocytes compared to control animals." [GO:0048477, WB:WBPerson2021]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
pachytene region organization variant

"Animals exhibit variations from control animals in the organization of the germ cell nuclei in the pachytene stage region of the gonad. In C. elegans the pachytene region begins just proximal to the germline's transition zone, where germ cells coordinately switch into the leptotene/zygotene stage from the premeiosis stage (WormAtlas)." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
pattern of transgene expression variant

"Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00071005   WBPaper00030951   Observed
rachis morphology variant

"Any variation in the form, structure or composition of the nucleus-free core of germ line cytoplasm in the gonad compared to control animals. In the C. elegans germline, nuclei exiting mitosis and entering meiosis are arranged along the cortex of the germline, partially enclosed by cellular membrane, forming a nucleus-free core of germline cytoplasm, called the rachis." [WB:WBPaper00031889, WB:WBPerson557]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
receptor mediated endocytosis defective

"Animals exhibit defects in the cell-mediated uptake of external materials via receptor activity. Receptor-mediated endocytosis ensures specificity of transport and generally occurs via clathrin-coated pits and vesicles. In C. elegans, this is often judged by the lack of yolk uptake in oocytes." [GO:0006898, pmid:16042554, WB:cab, WB:WBPerson2021]
RNAi: WBRNAi00071005   WBPaper00030951   Observed
slow growth

"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
RNAi: WBRNAi00000071   WBPaper00004402   Observed
sterile

"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
RNAi: WBRNAi00085831   WBPaper00038381   Observed
transgene subcellular localization variant

"Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00071753   WBPaper00030951   Observed


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