JCEG ID JCEG126
Name mig-22      WormBase    NCBI Gene
Human Ortholog

GGDB:CHPF 

OMIM: 608037 610405

OrthoList :   CHPF   CHPF2  (Ensembl Compara)

GGDB Score E-val
CHPF 187 9e-49
CSGlcA-T 151 3e-38
CSS3 64 9e-12
CSGALNACT1 49 4e-07
CHSY1 48 7e-07
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name Glycosaminoglycan (GAG ) and Chitin synthesis genes
Locus Tag PAR2.4
RNAi phenotype
phenotype Emb (100% n=13, see reference)
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
gg_motif:M1, M3, M4, M5, M6
Sequence CDS
Protein
References

Gonczy P, Echeverri C, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA Nature (2000) 408 (6810) :331-6 [PMID : 11099034] Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III.

Maeda I, Kohara Y, Yamamoto M, Sugimoto A Curr. Biol. (2001) 11 (3) :171-6 [PMID : 11231151] Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi.

Simmer F, Tijsterman M, Parrish S, Koushika SP, Nonet ML, Fire A, Ahringer J, Plasterk RH Curr. Biol. (2002) 12 (15) :1317-9 [PMID : 12176360] Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi.

Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.

Mizuguchi S, Uyama T, Kitagawa H, Nomura KH, Dejima K, Gengyo-Ando K, Mitani S, Sugahara K, Nomura K Nature (2003) 423 (6938) :443-8 [PMID : 12761550] Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans.

Izumikawa T, Kitagawa H, Mizuguchi S, Nomura KH, Nomura K, Tamura J, Gengyo-Ando K, Mitani S, Sugahara K J. Biol. Chem. (2004) 279 (51) :53755-61 [PMID : 15485872] Nematode chondroitin polymerizing factor showing cell-/organ-specific expression is indispensable for chondroitin synthesis and embryonic cell division.

Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ Nature (2005) 434 (7032) :462-9 [PMID : 15791247] Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

Suzuki N, Toyoda H, Sano M, Nishiwaki K Dev. Biol. (2006) 300 (2) :635-46 [PMID : 16982046] Chondroitin acts in the guidance of gonadal distal tip cells in C. elegans.

Balklava Z, Pant S, Fares H, Grant BD Nat. Cell Biol. (2007) 9 (9) :1066-73 [PMID : 17704769] Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic.

Kitagawa H, Izumikawa T, Mizuguchi S, Dejima K, Nomura KH, Egusa N, Taniguchi F, Tamura J, Gengyo-Ando K, Mitani S, Nomura K, Sugahara K J. Biol. Chem. (2007) 282 (11) :8533-44 [PMID : 17237233] Expression of rib-1, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis.

Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K Cell (2011) 145 (3) :470-82 [PMID : 21529718] A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

WormBase (WS254)

Functional Descriptions mig-22

Deletion Mutants k141 tk69
In Situ In Situ   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0004506

gon_herm_dtc_P "Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad" [ISBN:0-87969-307-X] Certain WB:Expr3103 WBPaper00024879
WB WBbt:0003681

pharynx "the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine." [WB:rynl] WB:Expr6441 WBPaper00006525 WormAtlas
WB WBbt:0004506

gon_herm_dtc_P "Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad" [ISBN:0-87969-307-X] Certain WB:Expr4271 WBPaper00028501
WB WBbt:0004520

gon_herm_dtc_A "Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad" [ISBN:0-87969-307-X] Certain WB:Expr3103 WBPaper00024879
WB WBbt:0004520

gon_herm_dtc_A "Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad" [ISBN:0-87969-307-X] Certain WB:Expr4271 WBPaper00028501
WB WBbt:0005751

coelomocyte "A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles." [WBPaper:00038216] Enriched WBPaper00037950 : coelomocytes_embryo_SelectivelyEnriched, coelomocytes_embryo_enriched
WBPaper00037950 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] WB:Expr6441 WBPaper00006525 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] Certain WB:Expr3103 WBPaper00024879 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] Certain WB:Expr4271 WBPaper00028501 WormAtlas
WB WBbt:0005784

germ line "cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes." [ISBN:0140512888] Enriched WBPaper00044760 : germline_specific
WBPaper00044760
WB WBbt:0006748

vulva "female genital." [WB:rynl] Certain WB:Expr3103 WBPaper00024879 WormAtlas
WB WBbt:0006748

vulva "female genital." [WB:rynl] Certain WB:Expr4271 WBPaper00028501 WormAtlas
WB WBbt:0006797

oocyte "a female haploid germ cell." [WB:wjc] Certain WB:Expr4271 WBPaper00028501 WormAtlas

WBGene00003253
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
chondroitin biosynthesis variant

"Animals exhibit variations in the chemical reactions and pathways resulting in the formation of chondroitin sulfate compared to control." [WB:WBPaper00029024, WB:WBPaper00031153, WB:WBPerson712]
RNAi: WBRNAi00061108   WBPaper00024879   Observed
cytokinesis defective early emb

"Cytokinesis is abnormal in the first or second stages of cell division." [WB:cab, WB:cgc7141]
RNAi: WBRNAi00077498   WBPaper00005868   Observed
early larval lethal

"Animals die during early larval development. In C.elegans, this occurs during the L1- L2 larval stages of the life cycle." [WB:WBPerson2021]
RNAi: WBRNAi00051081   WBPaper00025054   Observed
embryo osmotic integrity defective early emb

"Variations in the ability of an embryo to maintain osmotic balance. In C.elegans this can result in an embryo filling the egg shell and lysing." [WB:cab, WB:cgc7141]
RNAi: WBRNAi00051081   WBPaper00025054   Observed
RNAi: WBRNAi00051082   WBPaper00025054   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
RNAi: WBRNAi00002513   WBPaper00004403   Observed
RNAi: WBRNAi00002761   WBPaper00004403   Observed
RNAi: WBRNAi00001856   WBPaper00004651   Observed
germ cell compartment morphology variant

"Any variation in the form or composition of the compartments in the germline of females/hermaphrodites compared to control animals. In C. elegans these compartments contain a nucleus and are connected to a common cytoplasm (rachis). These compartments eventually undergo cellularization and become oocytes." [WB:WBPaper00038381, WB:WBPerson557]
RNAi: WBRNAi00085836   WBPaper00038381   Observed
heparan sulfate biosynthesis variant

"Animals exhibit variations in the chemical reactions and pathways resulting in the formation of the heparan sulfate proteoglycan compared to control." [WB:WBPaper00029024, WB:WBPaper00031153, WB:WBPerson712]
RNAi: WBRNAi00061108   WBPaper00024879   Observed
maternal sterile

" Animals produce no or very few embryos as a result of experimental treatment. This is in contrast to sterility due to genetic perturbations (maternal effect sterility)." [WB:cab, WB:cgc5599, WB:cgc7141, WB:WBPerson557]
RNAi: WBRNAi00051082   WBPaper00025054   Observed
multiple nuclei early emb

"Embryos contain more than one nucleus per cell in the inviable one- to four-cell embryo." [WB:cab, WB:cgc5599]
RNAi: WBRNAi00061108   WBPaper00024879   Observed
nuclear appearance variant

"The morphological appearance of nuclei differs from control animals." [WB:cab]
RNAi: WBRNAi00085836   WBPaper00038381   Observed
organism homeostasis metabolism variant

"Variations in the chemical reactions and pathways involved in the maintenance of a steady-state at the level of the organism compared to control." [GO:0048871, WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00061108   WBPaper00024879   Observed
pattern of transgene expression variant

"Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00071335   WBPaper00030951   Observed
protruding vulva

"Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva." [WB:WBPaper00004408, WB:WBPerson2021]
RNAi: WBRNAi00027422   WBPaper00006395   Observed
receptor mediated endocytosis defective

"Animals exhibit defects in the cell-mediated uptake of external materials via receptor activity. Receptor-mediated endocytosis ensures specificity of transport and generally occurs via clathrin-coated pits and vesicles. In C. elegans, this is often judged by the lack of yolk uptake in oocytes." [GO:0006898, pmid:16042554, WB:cab, WB:WBPerson2021]
RNAi: WBRNAi00071335   WBPaper00030951   Observed
sterile

"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
RNAi: WBRNAi00085836   WBPaper00038381   Observed


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