JCEG ID JCEG097
Name sqv-8      WormBase    NCBI Gene
Human Ortholog

GGDB:B3GAT3 B3GAT2 

OMIM: 151290 245600 606374 607497

OrthoList :   B3GAT3   B3GAT2   B3GAT1  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
UPSTREAM 0.000005 0.2918 (unit increase)] 23319000
MAPPED 0.219 0.000007 (ALT/AST) 0.03 (unit increase) [NR] 23251661
UPSTREAM 0.16 0.0000009 1.17 (unit decrease) [0.70-1.64] 21326311
GGDB Score E-val
B3GAT3 241 1e-65
B3GAT1 221 2e-59
B3GAT2 200 3e-53
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name Glucuronyltransferase(GlcAT)
Locus Tag ZK1307.5
RNAi phenotype
phenotype Emb (55% n=20, see reference)
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
dxd:DDD
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Sigurdson DC, Spanier GJ, Herman RK Genetics (1984) 108 (2) :331-45 [PMID : 6500256] Caenorhabditis elegans deficiency mapping.

Herman T, Horvitz HR Cold Spring Harb. Symp. Quant. Biol. (1997) 62 :353-9 [PMID : 9598369] Mutations that perturb vulval invagination in C. elegans.

Herman T, Hartwieg E, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (1999) 96 (3) :968-73 [PMID : 9927677] sqv mutants of Caenorhabditis elegans are defective in vulval epithelial invagination.

Herman T, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (1999) 96 (3) :974-9 [PMID : 9927678] Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway.

Bulik DA, Wei G, Toyoda H, Kinoshita-Toyoda A, Waldrip WR, Esko JD, Robbins PW, Selleck SB Proc. Natl. Acad. Sci. U.S.A. (2000) 97 (20) :10838-43 [PMID : 11005858] sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis.

Michaux G, Legouis R, Labouesse M Gene (2001) 277 (1-2) :83-100 [PMID : 11602346] Epithelial biology: lessons from Caenorhabditis elegans.

Johnson TE, Henderson S, Murakami S, de Castro E, de Castro SH, Cypser J, Rikke B, Tedesco P, Link C J. Inherit. Metab. Dis. (2002) 25 (3) :197-206 [PMID : 12137228] Longevity genes in the nematode Caenorhabditis elegans also mediate increased resistance to stress and prevent disease.

Hwang HY, Horvitz HR Proc. Natl. Acad. Sci. U.S.A. (2002) 99 (22) :14224-9 [PMID : 12391315] The Caenorhabditis elegans vulval morphogenesis gene sqv-4 encodes a UDP-glucose dehydrogenase that is temporally and spatially regulated.

Bulik DA, Robbins PW Biochim. Biophys. Acta (2002) 1573 (3) :247-57 [PMID : 12417407] The Caenorhabditis elegans sqv genes and functions of proteoglycans in development.

Sugahara K, Mikami T, Uyama T, Mizuguchi S, Nomura K, Kitagawa H Curr. Opin. Struct. Biol. (2003) 13 (5) :612-20 [PMID : 14568617] Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate.

Mizuguchi S, Uyama T, Kitagawa H, Nomura KH, Dejima K, Gengyo-Ando K, Mitani S, Sugahara K, Nomura K Nature (2003) 423 (6938) :443-8 [PMID : 12761550] Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans.

Sternberg PW WormBook (2005) :1-28 [PMID : 18050418] Vulval development.

Sato K, Sato M, Audhya A, Oegema K, Schweinsberg P, Grant BD Mol. Biol. Cell (2006) 17 (7) :3085-94 [PMID : 16672374] Dynamic regulation of caveolin-1 trafficking in the germ line and embryo of Caenorhabditis elegans.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Franks DM, Izumikawa T, Kitagawa H, Sugahara K, Okkema PG Dev. Biol. (2006) 296 (2) :409-20 [PMID : 16828468] C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans.

Hadwiger G, Dour S, Arur S, Fox P, Nonet ML PLoS ONE (2010) 5 (4) :e10161 [PMID : 20405020] A monoclonal antibody toolkit for C. elegans.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Berninsone PM, Hirschberg CB Glycoconj. J. 19 (4-5) :325-30 [PMID : 12975612] The nematode Caenorhabditis elegans as a model to study the roles of proteoglycans.

WormBase (WS254)

Functional Descriptions sqv-8

The sqv-8 gene encodes a glucuronyl transferase, biochemically active in vitro, that is required for cytokinesis of one-cell embryos and for vulval morphogenesis; SQV-8 is homologous to three distinct glucuronyl transferases (GlcAT-I, GlcAT-P and GlcAT-D) that play a role in the synthesis of different glycoconjugates; the common requirement for SQV-8 in both cytokinesis and morphogenesis may be to promote filling an extracellular space with hygroscopic proteoglycans (either in the eggshell, or underneath the L4 cuticle), which in turn may cause the space to fill with fluid.
Deletion Mutants mn63 n2822 n2825 n2843 n2847 n2850 n2851 n3036
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003679

neuron "Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses." [ISBN:0140512888] Enriched WBPaper00031532 : Larva_Pan_Neuronal_Enriched
WBPaper00031532 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Enriched WBPaper00037950 : hypodermis_larva_enriched
WBPaper00037950
WB WBbt:0006804

body wall muscle cell "type of 95 cells that make up muscles of the body wall." [WB:rynl] Enriched WBPaper00037950 : bodywall-muscle_larva_enriched
WBPaper00037950
WB WBbt:0006831

PVD "Neuron class of two interneurons with cell bodies situated laterally in the posterior body." [WB:Paper00000938] Enriched WBPaper00037950 : PVD-OLL-neurons_larva_enriched
WBPaper00037950
WB WBbt:0006847

OLL "Neuron class of two neurons with ciliated endings in the lateral outer labial sensilla." [WB:Paper00000938] Enriched WBPaper00037950 : PVD-OLL-neurons_larva_enriched
WBPaper00037950
WB WBbt:0006874

Psub1 "embryonic founder cell" [WB:rynl] Enriched WBPaper00046691 : P1_enriched
WBPaper00046691

WBGene00005026
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
bloated

"Worms appears turgid or swollen as a result of an excess number of eggs being retained in the uterus." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBVar00090577   WBPaper00003405   Observed
comma arrest emb

"Cessation of development during the middle stage in embryogenesis of the worm in which the embryo is slightly folded within the eggshell (Wormatlas)." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090577   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
Allele: WBVar00090594   WBPaper00003405   Observed
Allele: WBVar00090597   WBPaper00003405   Observed
Allele: WBVar00090600   WBPaper00003405   Observed
Allele: WBVar00090601   WBPaper00003405   Observed
hermaphrodite fertility reduced

"Hermaphrodites exhibit a reduction in the production of new individuals owing to defects in gamete competence and/or function compared to control animals." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBPerson261   WBVar00090580   Observed
hermaphrodite self sterile

"Hermaphrodites fail to produce any viable progeny via self-fertilization. However, such hermaphrodites can successfully reproduce if mated to control males." [WB:WBPaper00001075, WB:WBPerson2021]
Allele: WBPerson261   WBVar00089016   Observed
hermaphrodite sterile

"Hermaphrodites fail to generate viable self-progeny and/or cross-progeny." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBVar00090577   WBPaper00003405   Observed
larval arrest

"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
Allele: WBVar00089016   WBPaper00031153   Observed
Allele: WBVar00090580   WBPaper00031153   Observed
late embryonic arrest

"Cessation of development during the final stages of embryogenesis. In C. elegans, this stage normally occurs 620-800 min after the first cleavage at 20C and is the stage after elongation." [WB:WBPerson101]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
Allele: WBVar00090594   WBPaper00003405   Observed
Allele: WBVar00090597   WBPaper00003405   Observed
Allele: WBVar00090600   WBPaper00003405   Observed
Allele: WBVar00090601   WBPaper00003405   Observed
lethal

"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021]
Allele: WBVar00090577   WBPaper00003405   Observed
lumpy

"Animals have protrusions or bumps on their exterior which are not characteristic of control animals." [WB:WBPerson557]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
one cell arrest early emb

"Embryos fail to divide and arrest as one-cell embryos." [WB:cab, WB:cgc5599]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090577   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
Allele: WBVar00090594   WBPaper00003405   Observed
Allele: WBVar00090597   WBPaper00003405   Observed
Allele: WBVar00090600   WBPaper00003405   Observed
Allele: WBVar00090601   WBPaper00003405   Observed
oocyte morphology variant

"Haploid female germ cells (oocytes) exhibit variations in their overall structure, appearance or contents compared to control." [WB:WBPaper00001883, WB:WBPerson2021]
Allele: WBVar00090577   WBPaper00003405   Observed
pharyngeal development variant

"Variations in the progression of the pharynx (feeding organ) over time, from its formation to the mature structure compared to control. In C. elegans the pharynx is divided into the anterior and posterior regions. The anterior region includes the corpus (procorpus and metacorpus - first bulb) and the posterior region includes the isthmus and terminal bulb (second bulb)." [GO:0043282, WB:WBPerson2021]
Allele: WBVar00089016   WBPaper00031153   Observed
Allele: WBVar00090580   WBPaper00031153   Observed
squashed vulva

"The apparent partial collapse of the vulval invagination and the elongation of central vulva cells often leading to a flattened appearance." [WB:WBPaper00003405]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090577   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
Allele: WBVar00090594   WBPaper00003405   Observed
Allele: WBVar00090597   WBPaper00003405   Observed
Allele: WBVar00090600   WBPaper00003405   Observed
Allele: WBVar00090601   WBPaper00003405   Observed
sterile

"Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce." [GO:0000003, WB:WBPerson712]
Allele: WBVar00089016   WBPaper00003405   Observed
Allele: WBVar00090580   WBPaper00003405   Observed
Allele: WBVar00090594   WBPaper00003405   Observed
Allele: WBVar00090597   WBPaper00003405   Observed
Allele: WBVar00090600   WBPaper00003405   Observed
Allele: WBVar00090601   WBPaper00003405   Observed


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