JCEG ID JCEG089
Name cgt-2      WormBase    NCBI Gene
Human Ortholog

GGDB:UGCG 

OMIM: 602874

OrthoList :   UGCG  (Ensembl Compara)

GGDB Score E-val
UGCG 345 1e-96
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcT
Locus Tag F20B4.6
RNAi phenotype
phenotype
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features tm:soluble
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Ichikawa S, Hirabayashi Y Trends Cell Biol. (1998) 8 (5) :198-202 [PMID : 9695839] Glucosylceramide synthase and glycosphingolipid synthesis.

Leipelt M, Warnecke D, Zahringer U, Ott C, Muller F, Hube B, Heinz E J. Biol. Chem. (2001) 276 (36) :33621-9 [PMID : 11443131] Glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants, and fungi.

Lochnit G, Bongaarts R, Geyer R Int. J. Parasitol. (2005) 35 (8) :911-23 [PMID : 15885697] Searching new targets for anthelminthic strategies: Interference with glycosphingolipid biosynthesis and phosphorylcholine metabolism affects development of Caenorhabditis elegans.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Marza E, Simonsen KT, Faergeman NJ, Lesa GM J. Cell. Sci. (2009) 122 (Pt 6) :822-33 [PMID : 19240113] Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells.

Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K Glycobiology (2011) 21 (6) :834-48 [PMID : 21325339] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

WormBase (WS254)

Functional Descriptions cgt-2

cgt-2 encodes a transmembrane protein that is one of three C. elegans ceramide glucosyltransferases; by homology, CGT-2 is predicted to localize to the Golgi membrane and catalyze the transfer of glucose from UDP-glucose to ceramide to synthesize glucosylceramide, a glycosphingolipid precursor; although cgt-2 mutant worms show no obvious defects, cgt-3;cgt-1;cgt-2 triply mutant animals show reduced glucosylceramide levels; expression of cgt-2 in Saccharomyces cerevisiae results in de novo biosynthesis of glucosylceramides, absent from the parental strain.
Deletion Mutants tm1097 tm1192
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr11538 WBPaper00038174 WormAtlas
WB WBbt:0006748

vulva "female genital." [WB:rynl] Certain WB:Expr11538 WBPaper00038174 WormAtlas

WBGene00017625
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
reduced brood size

"The characteristic number of offspring produced is lower compared to that of control animals." [WB:WBPerson557]
RNAi: WBRNAi00083976   WBPaper00025177   Observed
RNAi: WBRNAi00083996   WBPaper00025177   Observed


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