JCEG ID JCEG082
Name gly-13      WormBase    NCBI Gene
Human Ortholog

GGDB:MGAT1 

OMIM: 160995

OrthoList :   POMGNT1  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
DOWNSTREAM 0.85 0.000006 (females) 0.16 (kg decrease) [NR] 19851299
DOWNSTREAM 0.85 0.00000007 (females) 2.96 (kg decrease) [NR] 19851299
MAPPED 0.47 0.000005 (HDL-C) 0.23 (mg/dL increase) [NR] 19197348
DOWNSTREAM 0.14 0.000008 (EA-glucose response) 3.67 (mg/dL increase) [2.06-5.28] 23400010
GGDB Score E-val
MGAT1 308 1e-85
POMGNT1 111 2e-26
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcNAcT(GnT)
Locus Tag B0416.6
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
dxd:EDD
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Chen S, Zhou S, Sarkar M, Spence AM, Schachter H J. Biol. Chem. (1999) 274 (1) :288-97 [PMID : 9867843] Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development.

Chen S, Tan J, Reinhold VN, Spence AM, Schachter H Biochim. Biophys. Acta (2002) 1573 (3) :271-9 [PMID : 12417409] UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans.

Zhang W, Cao P, Chen S, Spence AM, Zhu S, Staudacher E, Schachter H Biochem. J. (2003) 372 (Pt 1) :53-64 [PMID : 12603202] Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.

Zhu S, Hanneman A, Reinhold VN, Spence AM, Schachter H Biochem. J. (2004) 382 (Pt 3) :995-1001 [PMID : 15228383] Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I.

Paschinger K, Rendic D, Lochnit G, Jantsch V, Wilson IB J. Biol. Chem. (2004) 279 (48) :49588-98 [PMID : 15364955] Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans.

Shi H, Tan J, Schachter H Meth. Enzymol. (2006) 417 :359-89 [PMID : 17132514] N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Houston KM, Sutharsan R, Steiger CN, Schachter H, Harnett W Mol. Biochem. Parasitol. (2007) 157 (1) :88-91 [PMID : 17920136] Gene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis.

Butschi A, Titz A, Walti MA, Olieric V, Paschinger K, Nobauer K, Guo X, Seeberger PH, Wilson IB, Aebi M, Hengartner MO, Kunzler M PLoS Pathog. (2010) 6 (1) :e1000717 [PMID : 20062796] Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Chen S, Spence AM, Schachter H Biochimie 85 (3-4) :391-401 [PMID : 12770777] Isolation of null alleles of the Caenorhabditis elegans gly-12, gly-13 and gly-14 genes, all of which encode UDP-GlcNAc: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I activity.

WormBase (WS254)

Functional Descriptions gly-13

gly-13 encodes an experimentally verified UDP-N-acetylglucosamine alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I), that is the primary GnT I enzyme in vivo, and that can act on unusual substrates; gly-13 is expressed throughout development in many cell types; a deletion allele of gly-13 is partially lethal with survivors displaying severe morphological and behavioral defects.
Deletion Mutants ok712
In Situ In Situ   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003675

muscle cell "contractile cell that makes up muscle tissue." [WB:rynl] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0005135

head ganglion "ganglion in the head." [WB:rynl] Certain WB:Expr652 WBPaper00003352
WB WBbt:0005212

dorso-rectal ganglion "the ganglion that lies above and behind the rectum in the tail, in close continuity with the anal hypodermal ridge. It contains 3 neuron cell bodies (DVA, DVB and DVC) that send their neuronal processes into the ventral nerve cord via dorso-rectal commissures that encircle the anus. The ganglion contains no local neuropil in the hermaphrodite. In the adult male tail, this ganglion gains additional neurons and some local neuropil. " [WBPaper:00038216] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0005448

preanal ganglion "ganglion anterior to the anus." [WB:rynl] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Certain WB:Expr652 WBPaper00003352
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Enriched WBPaper00040985 : AIN-2_L4_intestine
WBPaper00040985 WormAtlas
WB WBbt:0005829

ventral nerve cord "a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring." [WB:Paper00000938] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0005830

lumbar ganglion "The left and right lumbar ganglia lie behind the pre-anal ganglion in the tail, in lateral positions. Each contains 12 neuron cell bodies that send most of their neuronal processes into the ventral nerve cord via lumbar commissures and the pre-anal ganglion. There is no local neuropil in these ganglia in the hermaphrodite, instead they form most synapses in the pre-anal ganglion. Most lumbar neurons have sensory functions. In the adult male tail, these two ganglia gain many additional neurons and have some local neuropil. The lumbar neurons lie in close association with the lateral hypodermis, and most are posterior to the anus." [WBPaper:00038216] Certain WB:Expr652 WBPaper00003352 WormAtlas
WB WBbt:0006748

vulva "female genital." [WB:rynl] Certain WB:Expr652 WBPaper00003352 WormAtlas

WBGene00001638
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
organism pathogen response variant

"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
RNAi: WBRNAi00065495   WBPaper00028824   Observed
pathogen induced death increased

"Animals are more prone to lethality as a result of infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00065494   WBPaper00028824   Observed


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