JCEG ID | JCEG081 | |||||||||
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Name | gly-14 WormBase NCBI Gene | |||||||||
Human Ortholog |
GGDB:MGAT1 OMIM: 160995 OrthoList : POMGNT1 (Ensembl Compara)
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Family Name | GlcNAcT(GnT) | |||||||||
Locus Tag | M01F1.1 | |||||||||
RNAi phenotype |
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Sequence Features |
kr:Detected tm:Detected dxd:EDD gg_motif:M1, M2, M3, M4, M5, M6 |
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Sequence | CDS |
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Protein |
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References |
Chen S, Zhou S, Sarkar M, Spence AM, Schachter H J. Biol. Chem. (1999) 274 (1) :288-97 [PMID : 9867843] Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development. Chen S, Tan J, Reinhold VN, Spence AM, Schachter H Biochim. Biophys. Acta (2002) 1573 (3) :271-9 [PMID : 12417409] UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans. Zhang W, Cao P, Chen S, Spence AM, Zhu S, Staudacher E, Schachter H Biochem. J. (2003) 372 (Pt 1) :53-64 [PMID : 12603202] Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase. Zhu S, Hanneman A, Reinhold VN, Spence AM, Schachter H Biochem. J. (2004) 382 (Pt 3) :995-1001 [PMID : 15228383] Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I. Paschinger K, Rendic D, Lochnit G, Jantsch V, Wilson IB J. Biol. Chem. (2004) 279 (48) :49588-98 [PMID : 15364955] Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans. Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation. Shi H, Tan J, Schachter H Meth. Enzymol. (2006) 417 :359-89 [PMID : 17132514] N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens. Houston KM, Sutharsan R, Steiger CN, Schachter H, Harnett W Mol. Biochem. Parasitol. (2007) 157 (1) :88-91 [PMID : 17920136] Gene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis. Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs. Chen S, Spence AM, Schachter H Biochimie 85 (3-4) :391-401 [PMID : 12770777] Isolation of null alleles of the Caenorhabditis elegans gly-12, gly-13 and gly-14 genes, all of which encode UDP-GlcNAc: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I activity. |
Functional Descriptions |
gly-14 gly-14 encodes a UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta-1, 2-N-acetylglucosaminyltransferase I (GnT 1); GLY-14 exhibits GnT 1 activity when assayed in vitro; a gly-14::lacZ reporter construct is expressed only in the intestine throughout larval and adult stages, with expression generally restricted to the anterior and posterior gut cells; a gly-14 deletion allele is phenotypically normal, even when in combination with a gly-12 mutation. |
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Deletion Mutants | id48 ok787 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In Situ | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Expression |
Expression
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WBGene00001639 Phenotype Homology_paralog |
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Phenotype | Definition | RNAi / Allele | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
pathogen resistance increased
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"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557] |
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transgene expression increased
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"Any increase in the intensity of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557] |
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