JCEG ID JCEG081
Name gly-14      WormBase    NCBI Gene
Human Ortholog

GGDB:MGAT1 

OMIM: 160995

OrthoList :   POMGNT1  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
DOWNSTREAM 0.85 0.000006 (females) 0.16 (kg decrease) [NR] 19851299
DOWNSTREAM 0.85 0.00000007 (females) 2.96 (kg decrease) [NR] 19851299
MAPPED 0.47 0.000005 (HDL-C) 0.23 (mg/dL increase) [NR] 19197348
DOWNSTREAM 0.14 0.000008 (EA-glucose response) 3.67 (mg/dL increase) [2.06-5.28] 23400010
GGDB Score E-val
MGAT1 304 2e-84
POMGNT1 130 6e-32
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name GlcNAcT(GnT)
Locus Tag M01F1.1
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
dxd:EDD
gg_motif:M1, M2, M3, M4, M5, M6
Sequence CDS
Protein
References

Chen S, Zhou S, Sarkar M, Spence AM, Schachter H J. Biol. Chem. (1999) 274 (1) :288-97 [PMID : 9867843] Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development.

Chen S, Tan J, Reinhold VN, Spence AM, Schachter H Biochim. Biophys. Acta (2002) 1573 (3) :271-9 [PMID : 12417409] UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans.

Zhang W, Cao P, Chen S, Spence AM, Zhu S, Staudacher E, Schachter H Biochem. J. (2003) 372 (Pt 1) :53-64 [PMID : 12603202] Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.

Zhu S, Hanneman A, Reinhold VN, Spence AM, Schachter H Biochem. J. (2004) 382 (Pt 3) :995-1001 [PMID : 15228383] Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I.

Paschinger K, Rendic D, Lochnit G, Jantsch V, Wilson IB J. Biol. Chem. (2004) 279 (48) :49588-98 [PMID : 15364955] Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Shi H, Tan J, Schachter H Meth. Enzymol. (2006) 417 :359-89 [PMID : 17132514] N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens.

Houston KM, Sutharsan R, Steiger CN, Schachter H, Harnett W Mol. Biochem. Parasitol. (2007) 157 (1) :88-91 [PMID : 17920136] Gene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

Chen S, Spence AM, Schachter H Biochimie 85 (3-4) :391-401 [PMID : 12770777] Isolation of null alleles of the Caenorhabditis elegans gly-12, gly-13 and gly-14 genes, all of which encode UDP-GlcNAc: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I activity.

WormBase (WS254)

Functional Descriptions gly-14

gly-14 encodes a UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta-1, 2-N-acetylglucosaminyltransferase I (GnT 1); GLY-14 exhibits GnT 1 activity when assayed in vitro; a gly-14::lacZ reporter construct is expressed only in the intestine throughout larval and adult stages, with expression generally restricted to the anterior and posterior gut cells; a gly-14 deletion allele is phenotypically normal, even when in combination with a gly-12 mutation.
Deletion Mutants id48 ok787
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0004355

int1VR "Intestinal cell" [ISBN:0-87969-307-X] WB:Expr654 WBPaper00003352
WB WBbt:0004356

int1DR "Intestinal cell" [ISBN:0-87969-307-X] WB:Expr654 WBPaper00003352
WB WBbt:0004360

int1VL "Intestinal cell" [ISBN:0-87969-307-X] WB:Expr654 WBPaper00003352
WB WBbt:0004361

int1DL "Intestinal cell" [ISBN:0-87969-307-X] WB:Expr654 WBPaper00003352
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] WB:Expr654 WBPaper00003352 WormAtlas
WB WBbt:0005784

germ line "cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes." [ISBN:0140512888] Enriched WBPaper00044760 : germline_specific
WBPaper00044760

WBGene00001639
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
pathogen resistance increased

"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
RNAi: WBRNAi00065496   WBPaper00028824   Observed
transgene expression increased

"Any increase in the intensity of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00094958   WBPaper00046421   Observed


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