JCEG ID |
JCEG064 |
Name |
piga-1
WormBase
NCBI Gene
|
Human Ortholog |
GGDB:PIGA
OMIM:
300818
300868
311770
OrthoList :
PIGA
(Ensembl Compara)
|
Family Name |
GPI-anchor synthesis |
Locus Tag |
D2085.6
|
RNAi phenotype |
phenotype
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Microscope observation (Image)
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COPAS (Image)
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hsp-4::GFP (Image)
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Sequence Features |
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Sequence |
CDS
ATGTCGCTAAAAATTGGTCCGTACAGTATTGCGTTGGTTTCCGATTTCTTCTGTCCAAAT GCTGGTGGAGTTGAAACTCATATCTACTTTCTTGCACAATGCCTCATCGAACTAGGGCAT CGAGTAGTAGTCATAACACATGGCTATGGAAATAGAAAAGGAATCAGATACTTGTCGAAT GGTCTAAAAGTCTATTATCTTCCATTTATTGTCGCTTATAATGGAGCTACATTGGGATCA ATTGTTGGAAGTATGCCGTGGCTACGGAAAGTTCTTTTGAGAGAAAATGTGCAGATAATT CATGGGCATTCGACATTTTCCTCGCTTGCACATGAAACACTGATGATTGGTGGCTTAATG GGGCTTCGAACAGTTTTCACTGATCACTCACTATTTGGATTTGCCGATGCTTCAGCTATT CTCACCAACAAATTGGTCCTACAATATTCTCTCATAAACGTAGATCAGACAATATGTGTG TCTTATACGAGTAAAGAAAATACAGTTTTACGCGGAAAGTTGGATCCAAACAAAGTGTCT ACAATACCAAATGCCATTGAGACATCACTGTTCACACCGGATCGTAATCAGTTTTTCAAC AATCCGACAACGATAGTGTTTCTAGGACGATTGGTATACCGGAAAGGAGCTGATCTTCTC TGTGAAATTGTGCCAAAGGTCTGCGCTCGTCACAAGTCGGTTCGCTTCATAATTGGCGGA GATGGTCCAAAACGTATCGAACTAGAAGAAATGCTCGAAAGATTCAAACTACACGAAAGA GTCGTTATTCTCGGAATGCTCCCCCATAATCAAGTGAAACGAGTCTTAAATCAAGGACAA ATTTTCATAAATACATCGCTTACTGAAGCGTTTTGTATGAGCATTGTTGAAGCCGCGAGT TGTGGTTTACATGTAGTCTCTACTCGAGTTGGAGGTGTTCCTGAAGTTCTTCCAATTGGA GAATTCATATCGTTAGAAGAACCAGTTCCAGATGATCTCGTTGATGCTCTTCTAAAAGCG GTTGATCGTCGAGAAAAAGGTCTATTGATGGATCCTACTGAAAAACATGAAGCTGTTTCA AAAATGTACAACTGGCCGGATGTAGCTGCAAGAACTCAAGTTATCTATCAAAAAGCCGTC GAGTCGGAGCCTACAGGAAGATTAGGAAGATTAAAAGGGTATTACGATCAAGGAATCGGG TTTGGAATCATGTATATCGTTGTTTCGTGTATCATAATTTTCTGGTTAACAGTCCTCGAT TTGTTTGATTCTCCAAGGAAAAACGGGACCAACGACAAAACGAGTGAGAAAAATGTTGAT CCTGACTATCAGTGA
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Protein
MSLKIGPYSIALVSDFFCPNAGGVETHIYFLAQCLIELGHRVVVITHGYGNRKGIRYLSN GLKVYYLPFIVAYNGATLGSIVGSMPWLRKVLLRENVQIIHGHSTFSSLAHETLMIGGLM GLRTVFTDHSLFGFADASAILTNKLVLQYSLINVDQTICVSYTSKENTVLRGKLDPNKVS TIPNAIETSLFTPDRNQFFNNPTTIVFLGRLVYRKGADLLCEIVPKVCARHKSVRFIIGG DGPKRIELEEMLERFKLHERVVILGMLPHNQVKRVLNQGQIFINTSLTEAFCMSIVEAAS CGLHVVSTRVGGVPEVLPIGEFISLEEPVPDDLVDALLKAVDRREKGLLMDPTEKHEAVS KMYNWPDVAARTQVIYQKAVESEPTGRLGRLKGYYDQGIGFGIMYIVVSCIIIFWLTVLD LFDSPRKNGTNDKTSEKNVDPDYQ
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References |
Piano F, Schetter AJ, Morton DG, Gunsalus KC, Reinke V, Kim SK, Kemphues KJ
Curr. Biol.
(2002)
12
(22)
:1959-64
[PMID : 12445391]
Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans.
Shaye DD, Greenwald I
PLoS ONE
(2011)
6
(5)
:e20085
[PMID : 21647448]
OrthoList: a compendium of C. elegans genes with human orthologs.
Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
Mol Biol Cell.
(2012)
23
(6)
:982-95
[PMID : 22298425]
GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans.
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Functional Descriptions |
piga-1
D2085.6 is orthologous to the human gene CLASS A GLCNAC-INOSITOL PHOSPHOLIPID ASSEMBLY PROTEIN (PIGA; OMIM:311770), which when mutated leads to paroxysmal nocturnal hemoglobinuria.
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Deletion Mutants |
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In Situ |
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Expression |
Expression
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WBGene00008431
Phenotype
Homology_paralog
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Phenotype |
Definition |
RNAi / Allele |
embryonic lethal
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"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
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high incidence male progeny
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"Higher numbers of male progeny occur in the population compared to control populations. In C. elegans, the high incidence of males phenotype in a self-fertilizing hermaphrodite population is a result of an increase in X chromosome nondisjunction." [WB:cab, WB:WBPaper00004402, WB:WBPaper00005654, WB:WBPerson712]
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larval arrest
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"The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage." [WB:WBPaper00002958, WB:WBPerson2021]
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larval lethal
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"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
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lethal
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"Animals die prematurely at any stage of the life cycle." [WB:WBPaper00001109, WB:WBPerson2021]
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maternal sterile
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" Animals produce no or very few embryos as a result of experimental treatment. This is in contrast to sterility due to genetic perturbations (maternal effect sterility)." [WB:cab, WB:cgc5599, WB:cgc7141, WB:WBPerson557]
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organism morphology variant
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"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395]
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postembryonic development variant
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"Any variation in the progression of an animal over time, from hatching to adulthood, compared to control." [WB:WBPerson2021]
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slow growth
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"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
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sterile progeny
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"Any variation that compromises the reproductive ability of the progeny of an animal." [WB:WBPerson2021, WB:WBPerson557]
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Variant
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"Animals exhibit variations compared to a given control." [WB:WBPerson557]
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