JCEG ID JCEG024
Name bus-4      WormBase
Human Ortholog

GGDB:C1GALT1 

OMIM:

OrthoList :   C1GALT1C1  (Ensembl Compara)

GWAS Catalog
STREAMRAFp-valueORBETACIStudy
DOWNSTREAM 0.0000002 (PCB105) 1.32 (unit decrease) [0.83-1.81] 25839716
MAPPED 0.46 0.000004 [NR] 19010793
MAPPED 0.444 0.000001 0.099 (unit decrease) [0.06-0.138] 24159190
MAPPED 0.444 0.0000006 0.101 (unit increase) [0.062-0.14] 24159190
GGDB Score E-val
C1GALT1 128 1e-31
C1GALT1C1 73 8e-15
CHSY1 60 6e-11
B3GLCT 57 4e-10
CSS3 49 1e-07
B3GNT4 49 1e-07
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name Core 1 GalT
Locus Tag T22B11.2
RNAi phenotype
phenotype WT
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)
Sequence Features kr:Detected
tm:Detected
dxd:DDD
gg_motif:M1,M3,M4,M6
Sequence CDS
Protein
References

Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms.

Samuelson AV, Carr CE, Ruvkun G Genes Dev. (2007) 21 (22) :2976-94 [PMID : 18006689] Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants.

Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans.

Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

WormBase (WS254)

Functional Descriptions bus-4

bus-4 encodes a galactosyltransferase; bus-4 is required for bacterial infection, and for fully normal cuticle integrity and body size; specifically, bus-4 is needed both for colonization by Microbacterium nematophilum (and the tail-swelling response to M. nematophilum infection) and for attachment of Yersinia pseudotuberculosis biofilms.
Deletion Mutants
In Situ
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0003681

pharynx "the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine." [WB:rynl] Partial WB:Expr9275 WBPaper00037686 WormAtlas
WB WBbt:0005733

hypodermis "epidermal layer" [WB:pws] Enriched WBPaper00037950 : hypodermis_embryo_SelectivelyEnriched, hypodermis_embryo_enriched
WBPaper00037950
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] Certain WB:Expr9275 WBPaper00037686 WormAtlas
WB WBbt:0005772

intestine "A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages." [WBPaper:00038216] Certain WB:Expr9275 WBPaper00037686 WormAtlas

WBGene00044620
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
age associated fluorescence increased

"Animals exhibit an increase in the intensity of fluorescence signals in intestinal cells with advancing age, compared to control." [WB:WBPaper00033040, WB:WBPerson2021]
RNAi: WBRNAi00070563   WBPaper00031212   Observed
bacterially unswollen

"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144924   WBPaper00026735   Observed
Allele: WBVar00144931   WBPaper00026735   Observed
Allele: WBVar00144932   WBPaper00026735   Observed
Allele: WBVar00144972   WBPaper00026735   Observed
Allele: WBVar00144997   WBPaper00026735   Observed
Allele: WBVar00145008   WBPaper00026735   Observed
Allele: WBVar00000541   WBPaper00029153   Observed
biofilm absent head

"Animals can move thorough a lawn of bacterially produced biofilm without accumulating an enormous amount of biofilm on its nose. When C. elegans is exposed to certain bacteria (e.g., Y. pestis), a biofilm accumulates on a worm's head. The presence of this biofilm inhibits feeding by the worm, and thereby prevents growth." [WB:cab, WB:WBPaper00005238]
Allele: WBVar00000541   WBPaper00029153   Observed
Allele: WBVar00144924   WBPaper00029153   Observed
cuticle carbohydrate surface variant

"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712]
Allele: WBVar00144932   WBPaper00026735   Observed
cuticle fragile

"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
Allele: WBVar00144924   WBPaper00026735   Observed
Allele: WBVar00144932   WBPaper00026735   Observed
Allele: WBVar00000541   WBPaper00029153   Observed
dauer lifespan extended

"The life span of dauer-staged animals is longer than that observed in control dauer animals." [WB:WBPaper00032396, WB:WBPerson2987]
RNAi: WBRNAi00103538   WBPaper00041478   Observed
organism hypersensitive desication

"Animals respond to less severe drought-like conditions or a shorter exposure compared to control animals." [WB:WBPerson2021]
Allele: WBVar00144932   WBPaper00026735   Observed
organism pathogen response variant

"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
Allele: WBVar00144924   WBPaper00037686   Observed
pathogen adherence defect

"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021]
Allele: WBVar00144924   WBPaper00026735   Observed
Allele: WBVar00144932   WBPaper00026735   Observed
pathogen resistance increased

"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBPerson261   WBVar00144924   Observed
post hermaphrodite contact variant

"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557]
Allele: WBVar00144924   WBPaper00037686   Observed
shortened life span

"The duration of viability in the adult phase of the life-cycle is less than that of control animals." [GO:0008340, WB:WBPerson712]
RNAi: WBRNAi00070560   WBPaper00031212   Observed
RNAi: WBRNAi00070562   WBPaper00031212   Observed
skiddy

"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144932   WBPaper00026735   Observed
Allele: WBVar00144924   WBPaper00037686   Observed
slow growth

"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
Allele: WBVar00144924   WBPaper00037686   Observed
small

"Animals are shorter and thinner than control animals at the same developmental stage." [WB:cab, WB:cgc31]
Allele: WBVar00144924   WBPaper00026735   Observed
Allele: WBVar00144932   WBPaper00026735   Observed
surface antigen negative

"Cuticles or cuticular extracts do not bind antibodies that are observed to bind the surfaces or cuticular extracts of Bristol N2 animals of the same developmental stage." [WB:WBPaper00001280, WB:WBPerson712]
Allele: WBVar00000541   WBPaper00029153   Observed


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