JCEG ID JCEG003
Name bus-8      WormBase    NCBI Gene
Human Ortholog

GGDB:ALG2 

OMIM:

OrthoList :   ALG2  (Ensembl Compara)

GGDB Score E-val
ALG2 168 2e-43
ALG11 63 8e-12
Option tblastn : Query Worm Protein, Subject GGDB mRNA, E-val < 0.00001
Family Name Asparagine-linked glycosylation (ALG) genes
Locus Tag T23F2.1
RNAi phenotype
phenotype small, skiddy, Dpy (100% n=33, see reference)
Microscope observation (Image)
COPAS (Image)

Normal

hsp-4::GFP (Image)

Normal

Sequence Features dxd:N.D.
tm:soluble
gg_motif:M1,M2
Sequence CDS
Protein
References

Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.

Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, Welchman DP, Zipperlen P, Ahringer J Nature (2003) 421 (6920) :231-7 [PMID : 12529635] Systematic functional analysis of the Caenorhabditis elegans genome using RNAi.

Nollen EA, Garcia SM, van Haaften G, Kim S, Chavez A, Morimoto RI, Plasterk RH Proc. Natl. Acad. Sci. U.S.A. (2004) 101 (17) :6403-8 [PMID : 15084750] Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation.

Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum.

Frand AR, Russel S, Ruvkun G PLoS Biol. (2005) 3 (10) :e312 [PMID : 16122351] Functional genomic analysis of C. elegans molting.

Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation.

Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms.

Samuelson AV, Carr CE, Ruvkun G Genes Dev. (2007) 21 (22) :2976-94 [PMID : 18006689] Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants.

Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans.

Partridge FA, Tearle AW, Gravato-Nobre MJ, Schafer WR, Hodgkin J Dev. Biol. (2008) 317 (2) :549-59 [PMID : 18395708] The C. elegans glycosyltransferase BUS-8 has two distinct and essential roles in epidermal morphogenesis.

Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans.

Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs.

McCormick AV, Wheeler JM, Guthrie CR, Liachko NF, Kraemer BC Biol. Psychiatry (2012) 73 (5) :464-71 [PMID : 23140663] Dopamine D2 receptor antagonism suppresses tau aggregation and neurotoxicity.

WormBase (WS254)

Functional Descriptions bus-8

bus-8 encodes a glycosyltransferase related to mannosyltransferases that play a role in protein N-linked glycosylation ; bus-8 is required for colonization by Microbacterium nematophilum and the tail-swelling response to M. nematophilum infection; bus-8 is also essential for enclosure during embryonic morphogenesis, functioning in or with ephrin-mediated signaling to effect the proper neuroblast organization that facilitates epidermal migration; bus-8 is also required for cuticle integrity, normal cuticle antigens, completely normal traction during movement, and viability; a BUS-8::GFP fusion protein is detected during embryonic morphogenesis in cells underlying the ventral epidermis (possibly neuroblasts), subsequent expression is then restricted to the hypodermal seam cells which continues throughout postembryonic development.
Deletion Mutants ok1175 tm1410 ok1176
In Situ In Situ   
Expression Expression
db_name wbbt name def reliability expr_pattern paper WormAtlas
WB WBbt:0004740

I5 neuron "Neuron class of one pharyngeal interneuron, posterior sensory." [ISBN:0-87969-307-X] Enriched WBPaper00025141 : unc-4
WBPaper00025141
WB WBbt:0005278

DA neuron "Neuron class of nine ventral cord motor neuron, innervates dorsal muscle." [WB:rynl] Enriched WBPaper00025141 : unc-4
WBPaper00025141
WB WBbt:0005278

DA neuron "Neuron class of nine ventral cord motor neuron, innervates dorsal muscle." [WB:rynl] Enriched WBPaper00030839 : Embryo_A_Class
WBPaper00030839
WB WBbt:0005396

SAB "Neuron class of three interneurons, which send processes anteriorly up the sub-lateral cords in the head." [WB:Paper00000938] Enriched WBPaper00025141 : unc-4
WBPaper00025141
WB WBbt:0005656

retrovesicular ganglion "ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.\nReversal \n\n" [WBPaper:00038216] Enriched WBPaper00025141 : unc-4
WBPaper00025141 WormAtlas
WB WBbt:0005753

seam cell "a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail" [WB:rynl, WBPaper:00038216] Certain WB:Expr8071 WBPaper00031667 WormAtlas

WBGene00044623
Phenotype
Homology_paralog

Observed Observed and NOT Observed
Phenotype Definition RNAi / Allele
age associated fluorescence increased

"Animals exhibit an increase in the intensity of fluorescence signals in intestinal cells with advancing age, compared to control." [WB:WBPaper00033040, WB:WBPerson2021]
RNAi: WBRNAi00070381   WBPaper00031212   Observed
bacterially unswollen

"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144929   WBPaper00026735   Observed
Allele: WBVar00088502   WBPaper00031667   Observed
Allele: WBVar00145031   WBPaper00031667   Observed
Allele: WBVar00145032   WBPaper00031667   Observed
Allele: WBVar00145033   WBPaper00031667   Observed
Allele: WBVar00145034   WBPaper00031667   Observed
Allele: WBVar00145038   WBPaper00031667   Observed
bleach hypersensitive

"Animals respond to bleach at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557]
Allele: WBPerson261   WBVar00144929   Observed
chemical response variant

"Animals exhibit variations in their response to a specific chemical compared to that observed in control animals." [WB:WBPerson557]
Allele: WBVar00145034   WBPaper00031667   Observed
cuticle carbohydrate surface variant

"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712]
Allele: WBVar00144929   WBPaper00026735   Observed
cuticle fragile

"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712]
Allele: WBVar00144929   WBPaper00026735   Observed
drug hypersensitive

"Animals respond to a specific drug at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557]
Allele: WBVar00088502   WBPaper00031667   Observed
Allele: WBVar00145031   WBPaper00031667   Observed
Allele: WBVar00145032   WBPaper00031667   Observed
Allele: WBVar00145033   WBPaper00031667   Observed
Allele: WBVar00145034   WBPaper00031667   Observed
Allele: WBVar00145038   WBPaper00031667   Observed
dumpy

"Worms are shorter and stouter than control animals at the same developmental stage." [WB:cab, WB:cgc31, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497]
RNAi: WBRNAi00026379   WBPaper00006395   Observed
embryonic lethal

"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054]
Allele: WBVar00145034   WBPaper00031667   Observed
Allele: WBVar00145038   WBPaper00031667   Observed
Allele: WBVar00145039   WBPaper00031667   Observed
Allele: WBVar00250403   WBPaper00031667   Observed
epithelial cell morphology variant

"Any variation in the morphological appearance of epithelial cells compared to control animals." [WB:WBPerson557]
Allele: WBVar00145034   WBPaper00031667   Observed
larval lethal

"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712]
RNAi: WBRNAi00009215   WBPaper00005654   Observed
Allele: WBVar00088502   WBPaper00031667   Observed
Allele: WBVar00145031   WBPaper00031667   Observed
Allele: WBVar00145032   WBPaper00031667   Observed
Allele: WBVar00145033   WBPaper00031667   Observed
Allele: WBVar00145034   WBPaper00031667   Observed
Allele: WBVar00145038   WBPaper00031667   Observed
locomotion variant

"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712]
RNAi: WBRNAi00026379   WBPaper00006395   Observed
molt defect

"Animals exhibit defects in the process of periodic shedding, of part or all of its cuticle, during cuticle replacement that punctuates each larval stage." [GO:0018996, WB:WBPerson712]
RNAi: WBRNAi00009215   WBPaper00005654   Observed
RNAi: WBRNAi00066814   WBPaper00026763   Observed
Allele: WBVar00145031   WBPaper00031667   Observed
Allele: WBVar00145034   WBPaper00031667   Observed
nicotine hypersensitive

"Animals respond to nicotine at lower concentrations or shorter exposure compared to control. Nicotine is a cholinergic agonist." [WB:cab, WB:cgc7388, WB:WBPerson557]
Allele: WBVar00088502   WBPaper00031667   Observed
organism morphology variant

"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395]
RNAi: WBRNAi00009215   WBPaper00005654   Observed
organism pathogen response variant

"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712]
Allele: WBVar00144929   WBPaper00037686   Observed
paralyzed

"Animals do not move and are not responsive to external mechanical stimulation." [WB:cab]
RNAi: WBRNAi00026379   WBPaper00006395   Observed
pathogen adherence defect

"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021]
Allele: WBVar00144929   WBPaper00026735   Observed
pathogen resistance increased

"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557]
Allele: WBPerson261   WBVar00144929   Observed
post hermaphrodite contact variant

"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557]
Allele: WBVar00144929   WBPaper00037686   Observed
protein aggregation variant

"Variations that result in the accumulation of soluble/insoluble protein aggregates in a particular cell or tissue, compared to control. Protein aggregates are often formed by interactions between misfolded protein molecules and/or deficiencies in proper protein folding." [WB:WBPaper00034765, WB:WBPerson2021]
RNAi: WBRNAi00065018   WBPaper00006510   Observed
protruding vulva

"Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva." [WB:WBPaper00004408, WB:WBPerson2021]
RNAi: WBRNAi00026379   WBPaper00006395   Observed
shortened life span

"The duration of viability in the adult phase of the life-cycle is less than that of control animals." [GO:0008340, WB:WBPerson712]
RNAi: WBRNAi00070378   WBPaper00031212   Observed
RNAi: WBRNAi00070379   WBPaper00031212   Observed
RNAi: WBRNAi00070380   WBPaper00031212   Observed
skiddy

"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735]
Allele: WBVar00144929   WBPaper00026735   Observed
Allele: WBVar00088502   WBPaper00031667   Observed
Allele: WBVar00145031   WBPaper00031667   Observed
Allele: WBVar00145032   WBPaper00031667   Observed
Allele: WBVar00145033   WBPaper00031667   Observed
Allele: WBVar00145034   WBPaper00031667   Observed
Allele: WBVar00145038   WBPaper00031667   Observed
slow growth

"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557]
RNAi: WBRNAi00026379   WBPaper00006395   Observed
transgene subcellular localization variant

"Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557]
RNAi: WBRNAi00065018   WBPaper00006510   Observed
ventral enclosure variant

"Any variation in the process that regulates the migration of ventral epidermal cells toward the ventral midline (to encase underlying cells in an epithelial monolayer) in developing embryos compared to control." [WB:WBPaper00027244, WB:WBPerson2021]
Allele: WBVar00250403   WBPaper00031667   Observed


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