JCEG ID | JCEG003 | |||||||||
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Name | bus-8 WormBase NCBI Gene | |||||||||
Human Ortholog |
GGDB:ALG2 OMIM: OrthoList : ALG2 (Ensembl Compara)
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Family Name | Asparagine-linked glycosylation (ALG) genes | |||||||||
Locus Tag | T23F2.1 | |||||||||
RNAi phenotype |
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Sequence Features |
dxd:N.D. tm:soluble gg_motif:M1,M2 |
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Sequence | CDS |
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Protein |
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References |
Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH PLoS Biol. (2003) 1 (1) :E12 [PMID : 14551910] Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, Welchman DP, Zipperlen P, Ahringer J Nature (2003) 421 (6920) :231-7 [PMID : 12529635] Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nollen EA, Garcia SM, van Haaften G, Kim S, Chavez A, Morimoto RI, Plasterk RH Proc. Natl. Acad. Sci. U.S.A. (2004) 101 (17) :6403-8 [PMID : 15084750] Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Gravato-Nobre MJ, Nicholas HR, Nijland R, O'Rourke D, Whittington DE, Yook KJ, Hodgkin J Genetics (2005) 171 (3) :1033-45 [PMID : 16079230] Multiple genes affect sensitivity of Caenorhabditis elegans to the bacterial pathogen Microbacterium nematophilum. Frand AR, Russel S, Ruvkun G PLoS Biol. (2005) 3 (10) :e312 [PMID : 16122351] Functional genomic analysis of C. elegans molting. Berninsone PM WormBook (2006) :1-22 [PMID : 18050430] Carbohydrates and glycosylation. Darby C, Chakraborti A, Politz SM, Daniels CC, Tan L, Drace K Genetics (2007) 176 (1) :221-30 [PMID : 17339204] Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms. Samuelson AV, Carr CE, Ruvkun G Genes Dev. (2007) 21 (22) :2976-94 [PMID : 18006689] Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants. Drace K, Darby C Appl. Environ. Microbiol. (2008) 74 (14) :4509-15 [PMID : 18515487] The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans. Partridge FA, Tearle AW, Gravato-Nobre MJ, Schafer WR, Hodgkin J Dev. Biol. (2008) 317 (2) :549-59 [PMID : 18395708] The C. elegans glycosyltransferase BUS-8 has two distinct and essential roles in epidermal morphogenesis. Gravato-Nobre MJ, Stroud D, O'Rourke D, Darby C, Hodgkin J Genetics (2010) 187 (1) :141-55 [PMID : 20980242] Glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of Caenorhabditis elegans. Shaye DD, Greenwald I PLoS ONE (2011) 6 (5) :e20085 [PMID : 21647448] OrthoList: a compendium of C. elegans genes with human orthologs. McCormick AV, Wheeler JM, Guthrie CR, Liachko NF, Kraemer BC Biol. Psychiatry (2012) 73 (5) :464-71 [PMID : 23140663] Dopamine D2 receptor antagonism suppresses tau aggregation and neurotoxicity. |
Functional Descriptions |
bus-8 bus-8 encodes a glycosyltransferase related to mannosyltransferases that play a role in protein N-linked glycosylation ; bus-8 is required for colonization by Microbacterium nematophilum and the tail-swelling response to M. nematophilum infection; bus-8 is also essential for enclosure during embryonic morphogenesis, functioning in or with ephrin-mediated signaling to effect the proper neuroblast organization that facilitates epidermal migration; bus-8 is also required for cuticle integrity, normal cuticle antigens, completely normal traction during movement, and viability; a BUS-8::GFP fusion protein is detected during embryonic morphogenesis in cells underlying the ventral epidermis (possibly neuroblasts), subsequent expression is then restricted to the hypodermal seam cells which continues throughout postembryonic development. |
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Deletion Mutants | ok1175 tm1410 ok1176 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In Situ | In Situ | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Expression |
Expression
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WBGene00044623 Phenotype Homology_paralog |
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Phenotype | Definition | RNAi / Allele | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
age associated fluorescence increased
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"Animals exhibit an increase in the intensity of fluorescence signals in intestinal cells with advancing age, compared to control." [WB:WBPaper00033040, WB:WBPerson2021] |
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bacterially unswollen
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"Animals do not exhibit tail-swelling in response to infection by a bacterial pathogen (such as Microbacterium nematophilum) compared to control." [WB:cab, WB:WBPaper00026735] |
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bleach hypersensitive
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"Animals respond to bleach at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557] |
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chemical response variant
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"Animals exhibit variations in their response to a specific chemical compared to that observed in control animals." [WB:WBPerson557] |
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cuticle carbohydrate surface variant
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"Animals exhibit an altered display of glycosylated proteins or lipids or altered access to glycosylated moieties along their surface from that observed in control animals of the same developmental stage. In C. elegans, variations in surface accessible glycosylated species are often revealed by altered lectin-binding from that observed in control animals." [WB:WBPaper00001576, WB:WBPerson712] |
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cuticle fragile
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"The strength of the cuticle is less robust than that observed for control animals. In C. elegans the strength of the cuticle is often assayed by it's ability to protect the animal from exposure to bleach or other compounds." [WB:WBPerson712] |
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drug hypersensitive
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"Animals respond to a specific drug at a lower concentration or a shorter exposure compared to control animals." [WB:WBPerson557] |
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dumpy
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"Worms are shorter and stouter than control animals at the same developmental stage." [WB:cab, WB:cgc31, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497] |
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embryonic lethal
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"Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die." [WB:cab, WB:WBPaper00000179, WB:WBPaper00004403, WB:WBPaper00004540, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00024497, WB:WBPaper00024925, WB:WBPaper00025054] |
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epithelial cell morphology variant
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"Any variation in the morphological appearance of epithelial cells compared to control animals." [WB:WBPerson557] |
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larval lethal
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"The animal dies at any time between hatching and onset to adulthood." [GO:0002119, WB:WBPerson712] |
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locomotion variant
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"Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals." [GO:0040011, WB:cab, WB:WBPaper00024949, WB:WBPerson712] |
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molt defect
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"Animals exhibit defects in the process of periodic shedding, of part or all of its cuticle, during cuticle replacement that punctuates each larval stage." [GO:0018996, WB:WBPerson712] |
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nicotine hypersensitive
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"Animals respond to nicotine at lower concentrations or shorter exposure compared to control. Nicotine is a cholinergic agonist." [WB:cab, WB:cgc7388, WB:WBPerson557] |
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organism morphology variant
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"Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00005654, WB:WBPaper00006395] |
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organism pathogen response variant
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"Animals exhibit variations in their response to disease causing agents, compared to control. Pathogen response includes prevention, damage control and recovery from the infection caused by the attack." [GO:0006952, WB:WBPaper00032196, WB:WBPerson2021, WB:WBPerson712] |
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paralyzed
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"Animals do not move and are not responsive to external mechanical stimulation." [WB:cab] |
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pathogen adherence defect
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"Pathogens are unable to adhere/colonize the surface of host animals. This defect is often associated with changes in cuticle surface properties of the host." [WB:WBPaper00028877, WB:WBPerson2021] |
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pathogen resistance increased
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"Animals are less prone to infection compared to control." [WB:WBPerson2021, WB:WBPerson557] |
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post hermaphrodite contact variant
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"Males exhibit variations in mating behavior after contacting its mate compared to control." [WB:WBPerson557] |
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protein aggregation variant
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"Variations that result in the accumulation of soluble/insoluble protein aggregates in a particular cell or tissue, compared to control. Protein aggregates are often formed by interactions between misfolded protein molecules and/or deficiencies in proper protein folding." [WB:WBPaper00034765, WB:WBPerson2021] |
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protruding vulva
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"Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva." [WB:WBPaper00004408, WB:WBPerson2021] |
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shortened life span
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"The duration of viability in the adult phase of the life-cycle is less than that of control animals." [GO:0008340, WB:WBPerson712] |
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skiddy
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"Apparently normal sinusoidal movement, but appear to have poor traction on the agar surface of a culture plate." [WB:cab, WB:WBPaper00026735] |
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slow growth
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"Any variation that causes a reduction in growth rate compared to control." [WB:cab, WB:WBPaper00004402, WB:WBPaper00004403, WB:WBPaper00004651, WB:WBPaper00004769, WB:WBPaper00005654, WB:WBPaper00006395, WB:WBPaper00024497, WB:WBPerson557] |
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transgene subcellular localization variant
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"Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals." [WB:WBPerson557] |
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ventral enclosure variant
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"Any variation in the process that regulates the migration of ventral epidermal cells toward the ventral midline (to encase underlying cells in an epithelial monolayer) in developing embryos compared to control." [WB:WBPaper00027244, WB:WBPerson2021] |
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