Mannan-binding protein (MBP), also called mannose-binding protein (MBP) or mannan-binding lectin (MBL), is a Ca2+-dependent (C-type) mammalian lectin specific for mannose, N-acetylglucosamine and fucose. MBP activates complement through the lectin pathway, and is an important serum component associated with innate immunity (Kawasaki T. 1999). MBP also binds to human colorectal carcinoma cells.
This chart shows the relation between a family of mannan-binding protein (MBP)-associated protocols.
- The methods for the isolation of MBP
Isolation of mannan-binding protein from human serum (plasma) ~Method 1
Isolation of mannan-binding protein from human serum (plasma) ~Method 2
Isolation of mannan-binding protein from human serum (plasma) ~Method 3
- The methods for the estimation of the lectin activity of the purified MBP
Assay method for the lectin activity of mannan-binding protein ~ELLBA-1*
Assay method for the lectin activity of mannan-binding protein ~ELLBA-2
Assay method for the lectin activity of mannan-binding protein ~ELLBA-3
*PV-Man used in ELLBA-1 is not commercially available at present.
- The method for MBP protein determination
Determination of mannan-binding protein by enzyme-linked immunosorbent assaying (ELISA)
This method can be used for the estimation of the MBP content not only in the purified samples
but also in the crude samples like human serum or plasma.
Characteristics of each method were evaluated in a 3-point scale (see below).
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| Category | Sugar binding proteins |
| Protocol Name | Determination of mannan-binding protein by enzyme-linked immunosorbent assay (ELISA) |
Authors
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Kawasaki, Nobuko
Research Center for Glycobiotechnology, Ritsumeikan University
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| KeyWords |
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| Methods |
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Determination of mannan-binding protein by enzyme-linked immunosorbent assaying (ELISA) |
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An assay kit (MBP-oligomer ELISA, Microwell strip version) for the determination of MBP is commercially available from BioPorto Diagnostics A/S (Gentofte, Denmark). The kit contains strips of microwells precoated with mouse anti-human MBP monoclonal antibody, buffers, standards (5, 10, 20, and 40 ng purified MBP/mL), biotinylated anti-human MBP antibody, streptavidin-HRP, a substrate solution containing tetramethylbenzidine (TMBZ), and a stop solution. The amount of MBP, principally an oligomer, in human serum or plasma, or a MBP preparation purified from human serum or plasma can be determined with reference to the purified MBP in the kit according to the manufacturer's instructions. Optimum measurement range: 50 ng/mL to 2500 ng/mL in undiluted samples. Detection limit: 5 ng/mL (diluted sample). |
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| Date of registration：2015-02-25 13:33:47 |
- Kawasaki, T. (1999) Structure and biology of mannan-binding protein, MBP, an important component of innate immunity. Biochim Biophys Acta 1473, 186–195 [PMID : 10580138]
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