Assay method for the lectin activity of mannan-binding protein ~ELLBA-3

Mannan-binding protein (MBP), also called mannose-binding protein (MBP) or mannan-binding lectin (MBL), is a Ca2+-dependent (C-type) mammalian lectin specific for mannose, N-acetylglucosamine and fucose. MBP activates complement through the lectin pathway, and is an important serum component associated with innate immunity (Kawasaki T. 1999). MBP also binds to human colorectal carcinoma cells.

This chart shows the relation between a family of mannan-binding protein (MBP)-associated protocols.

• The methods for the isolation of MBP

                Isolation of mannan-binding protein from human serum (plasma) ~Method 1
                Isolation of mannan-binding protein from human serum (plasma) ~Method 2
                Isolation of mannan-binding protein from human serum (plasma) ~Method 3

• The methods for the estimation of the lectin activity of the purified MBP

                Assay method for the lectin activity of mannan-binding protein ~ELLBA-1*
                Assay method for the lectin activity of mannan-binding protein ~ELLBA-2
                Assay method for the lectin activity of mannan-binding protein ~ELLBA-3

                    *PV-Man used in ELLBA-1 is not commercially available at present.

• The method for MBP protein determination

                Determination of mannan-binding protein by enzyme-linked immunosorbent assaying (ELISA)

                    This method can be used for the estimation of the MBP content not only in the purified samples

                    but also in the crude samples like human serum or plasma.

Characteristics of each method were evaluated in a 3-point scale (see below).

1. Kawasaki, T. (1999) Structure and biology of mannan-binding protein, MBP, an important component of innate immunity. Biochim Biophys Acta 1473, 186–195 [PMID : 10580138]
2. Haurum, J.S., Thiel, S., Haagsman, H.P., Laursen, S.B., Larsen, B., and Jensenius, J.C. (1993) Studies on the carbohydrate-binding characteristics of human pulmonary surfactant-associated protein A and comparison with two other collectins: mannan-binding protein and conglutinin. Biochem J. 293, 873–878 [PMID : 8352755]