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Behavioral analysis in Drosophila melanogaster
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Behavioral analysis in Drosophila melanogaster

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Introduction Protocol References Credit lines
Category
Glycogene transgenic animals
Protocol Name

Behavioral analysis in Drosophila melanogaster

Authors
 Ueyama, Morio
Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University

Nishihara, Shoko *
Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University
*To whom correspondence should be addressed.
KeyWords
Reagents
●

Mineral oil

Instruments
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1 mL-Syringe (58 mm tall and 6 mm in diameter: we use TERMO® syringe. Ca.#SS-01T)
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Absorbent cotton
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Glass measuring cylinder (2000 mL)
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Funnel
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Glass vials (240 mm tall and 25 mm in diameter)
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Digital camera
Methods
1.

Locomotion assay

1) 

 Put a fly in the syringe gently (see Comment).

 Comment 1
2) 

 After a 5-min rest, score the number of times the fly crossed the center (0.5 mL mark) of the syringe in the next 5 min.

 Comment 0
3) 

 Perform 10–20 individuals, and calculate the average times the fly crossed.

 Comment 0
2.

Flight assay

1) 

 Dump twenty individual flies through a funnel into a glass cylinder of which inside surface is coated with mineral oil.

 Comment 0
2) 

 Record the scores individually, and calculate the scores average from 5 independent experiments (see Comment).

 Comment 1
3.

Climbing assay

1) 

 Introduce twenty individual flies into a glass vial gently (see Comment).

 Comment 1
2) 

 After a 5-min rest, tap the bottom of the vial.

 Comment 0
3) 

 Record the height reached in 10 sec by a digital camera.

 Comment 0
4) 

 Perform five trials in each experiment, and calculate climbing ability (average ± standard error) from the 5 trials.

 Comment 0
Notes

The flies are reared at 25 degree using a cornmeal-yeast-glucose standard medium under natural light conditions. Adult flies are collected within 8 h of eclosion. Every two days, adult flies are transferred a fresh food vial. All behavioral experiments should be done during daytime. 

Figure & Legends

Figure & Legends

 

 

 

Fig. 1. Tools for locomotion assay

 

 

 

Fig. 2. Tools for flight assay

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